摘要
目的:建立同时测定保健食品中汉黄岑苷、4’-羟基汉黄岑素、去甲汉黄岑素、黄岑素、汉黄岑素、白杨素的分析方法。方法:样品经丙酮提取,C_(18)固相萃取柱净化,采用高效液相色谱仪,以样品基质匹配的标准曲线法进行定量分析。色谱柱为Phenomenex Luna C_(18)(250 mm×4.6 mm,5μm),通过流动相(乙腈—乙酸溶液)进行梯度洗脱,流速为1.0 mL/min,柱温为30℃,检测波长为280 nm。结果:保健食品基质中6种目标物分离情况良好,相应的浓度与峰面积呈良好的线性关系,相关系数(R 2)均>0.99,精密度和稳定性试验RSD≤3.08%,加标平均回收率为83%~100%(RSD<4.63%)。方法检出限为0.02~0.07 mg/kg,方法定量限为0.08~0.25 mg/kg。结论:该方法重现性好、有机溶剂消耗少,适用于保健食品中6种黄酮类化合物的同时测定。
Objective:To establish a method for simultaneous determination of wogonoside,4'-hydroxywogonin,norwogonin,baicalein,wogonin and chrysin in health care products by high performance liquid chromatography(HPLC).Methods:The samples were extracted with acetone,C_(18) solid phase extraction column was used for purification.The target compounds were determined by HPLC,quantified by matrix-matched standard curve method.Phenomenex Luna C_(18)(250 mm×4.6 mm,5μm)column was applied,mobile phase was acctonitrile-acetic acid,using gradient elution)at the flow rate of 1.0 mL/min.The temperature of column was 30℃and the detection wavelength was set at 280 nm.Results:The results showed that these 6 target compounds had good separation and the corresponding concentration had a good linear relationship with the peak area.Correlation coefficients(R 2)were all greater than 0.99,RSDs of precision and stability tests were within 3.08%and the average recoveries were 83%~100%(RSD<4.63%).The limits of detection were 0.02~0.07 mg/kg.The limits of quantification were 0.08~0.25 mg/kg.Conclusion:This method is suitable for simultaneous determination of flavonoids in health care products with good reproducibility,and less consumption of organic solvents.
作者
伍依琪
梁志森
陈玉珍
周睿
林晓君
WU Yi-qi;LIANG Zhi-sen;CHEN Yu-zhen;ZHOU Rui;LIN Xiao-jun(Guangzhou Inspection and Testing Certification Group Company Limited,Guangzhou,Guangdong 511447,China;National Quality Inspection and Testing Center for Processed Food〔Guangdong〕,Guangzhou,Guangdong 511447,China)
出处
《食品与机械》
北大核心
2022年第8期70-75,共6页
Food and Machinery
基金
广东省质量技术监督局科技项目(编号:2020CS02)。
关键词
高效液相色谱法
黄酮类化合物
保健食品
high performance liquid chromatography
flavonoids
health care products
