摘要
烟草青枯病是由茄科雷尔氏菌引起的一种细菌性土传病害,针对该青枯病菌建立了一套二次荧光定量PCR方法,对青枯病菌DNA的检测灵敏度可达0.06 pg/μL。对广西百色等地烟区201份土壤样品中的青枯病菌进行定量检测,结果表明发病土壤中青枯病菌含量明显高于未发病土壤,前者含有青枯病菌的阈值(CT值)为15.6~20.5,后者含有青枯病菌的阈值(CT值)为20.5~24.7。本研究建立的二次荧光定量PCR方法可以快速、准确地检测土壤中青枯病菌的丰度,为烟草青枯病预警提供技术支持。
Tobacco bacterial wilt is a soil-borne bacterial disease caused by Ralstonia solanacearum.In this study,we established a secondary fluorescence quantitative PCR method for R.solanacearum.The detection sensitivity of R.solanacearum DNA can reach 0.06 pg/μL.Quantitative detection of R.solanacearum in 201 soil samples from the tobacco planting area in Baise of Guangxi showed that the content of R.solanacearum in the infected soil was significantly higher than that in non-infected soil,and the threshold value(CT value)of R.solanacearum in diseased soil and healthy soil were 15.6~20.5 and 20.5~24.7,respectively.In summary,the secondary fluorescence quantitative PCR detection method established in this experiment can quickly and accurately detect the abundance of R.solanacearum in the soil,which provides technical support for the detection and early warning of tobacco bacterial wilt.
作者
何子康
张纪利
聂锦瑶
齐是
彭玮瑶
黎平
刘桔
韦建玉
颜健
He Zikang;Zhang Jili;Nie Jinyao;Qi Shi;Peng Weiyao;Li Ping;Liu Ju;Wei Jianyu;Yan Jian(College of Natural Resources and Environment/Key Laboratory of Agro-Environment in the Tropics,Ministry of Agriculture and Rural Affairs/Guangdong Provincial Key Laboratory of Eco-Circular Agriculture/Guangdong Engineering Research Centre for Modern Eco-Agriculture,South China Agricultural University,Guangzhou Guangdong 510642,China;China Tobacco Guangxi Industrial Co.Ltd.,Nanning Guangxi 530001,China;Guangdong Provincial Tobacco Shaoguan Co.Ltd.,Shaoguan Guangdong 512000,China)
出处
《中国植保导刊》
北大核心
2022年第8期5-9,14,共6页
China Plant Protection
基金
土壤中主要烟草根茎病害病原微生物检测及防治技术研究(2020450000340022)。
