摘要
MAPKKs是促丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)级联反应主要成员之一,位于该级联反应通路中间,对信号传递起着收集和发散的关键作用。有研究表明马铃薯StMAPKK4基因响应干旱胁迫,为进一步探究该基因的生物学功能,本研究对StMAPKK4基因进行了生物信息学分析。结果表明,StMAPKK4与科民茄亲缘关系最近。其含有蛋白激酶家族的Protein kinase domain(PF00069)结构域,位置定位于64~302 aa之间。StMAPKK4含有多个激素(茉莉酸甲酯、乙烯、脱落酸、ABA)及胁迫相关响应元件。qRT-PCR分析结果表明,StMAPKK4基因在马铃薯茎中表达量最高,且在干旱及盐处理下均上调表达。亚细胞定位表明该基因定位于细胞膜上。利用酵母双杂交方法筛选出8个与StMAPKK4相互作用的蛋白,并通过回转试验验证了其互作的真实性。对互作蛋白进行Blast比对结果显示,StMAPKK4与多酚氧化酶、藻蓝蛋白、天冬氨酸转氨酶、渗透素、磷酸盐转运蛋白等多种蛋白互作,初步判断StMAPKK4可能参与光合作用响应机制、植物体低温、干旱及盐胁迫等一系列非生物胁迫、促进根系对磷元素的吸收等生理生化过程。
MAPKKs,one of the main members of mitogen-activated protein kinase(MAPK)cascade,are located in the middle of the cascade pathway and play a key role in signal transduction.Studies have shown that potato StMAPKK4 gene responds to drought stress.To further explore the biological function of StMAPKK4 gene,bioinformatics analysis was conducted in the study.The results showed that StMAPKK4 was most closely related to Solanum commersonii.It contained the protein kinase domain(PF00069)of the protein kinase family,which located between 64 aa and 302 aa.StMAPKK4 contained multiple hormones(methyl jasmonate,ethylene,and abscisic acid)and stress-related response elements.The qRT-PCR analysis revealed that StMAPKK4 gene expression was the highest in potato stem.The relative expression levels were up-regulated under drought and salt treatments.Subcellular localization indicated that StMAPKK4 was localized on the cell membrane.Furthermore,eight proteins interacting with StMAPKK4 were screened by yeast two-hybrid method,and their interaction was verified by rotation experiment.Blast comparison of the interacting proteins indicated that StMAPKK4 interacted with polyphenol oxidase,phycocyanin,aspartate aminotransferase,osmotin,phosphate transporter,and other proteins.It was preliminarily concluded that StMAPKK4 may be involved in the response mechanism of photosynthesis,a series of abiotic stresses such as low temperature,drought,and salt stresses in plants,and promote the uptake of phosphorus in roots.
作者
濮雪
王凯彤
张宁
司怀军
PU Xue;WANG Kai-Tong;ZHANG Ning;SI Huai-Jun(College of Life Science and Technology,Gansu Agricultural University,Lanzhou 730070,Gansu,China;State Key Laboratory of Aridland Crop Science,Gansu Agricultural University,Lanzhou 730070,Gansu,China)
出处
《作物学报》
CAS
CSCD
北大核心
2023年第1期36-45,共10页
Acta Agronomica Sinica
基金
国家自然科学基金项目(31960444)
甘肃省干旱生境作物学重点实验室——省部共建国家重点实验室培育基地主任基金课题(GSCS-2019-Z03)资助。
