摘要
目的筛选人参-茯苓药对抗衰老的最佳配伍比例及给药条件,并探讨其作用机制。方法制备不同配伍比例(1∶1、1∶2、2∶1、1∶4、4∶1,m/m)的人参-茯苓药对提取物,并以酿酒酵母为衰老模型生物,采用MTT法绘制酿酒酵母生长曲线,筛选人参-茯苓药对的最佳配伍比例、给药浓度和给药时间点,检测酿酒酵母细胞内抗氧化酶[超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)]活性和活性氧(ROS)、丙二醛(MDA)水平以及三磷酸腺苷(ATP)含量和线粒体膜电位(MMP),检测酿酒酵母细胞内SOD1、CTT1、GSH1、ATP1、MRS1、CDC19 mRNA的表达水平。结果人参-茯苓药对抗衰老的最佳配伍比例为1∶4(m/m),最佳给药浓度为220μg/mL,最佳给药时间点为第28 h。人参-茯苓药对1∶4提取物可显著升高酿酒酵母细胞内SOD、POD、CAT活性,ATP含量和MMP以及CTT1、GSH1、MRS1 mRNA表达水平(P<0.01),显著降低MDA、ROS水平和SOD1、ATP1、CDC19 mRNA表达水平(P<0.05或P<0.01)。结论人参-茯苓药对以1∶4(m/m)配伍时对酿酒酵母的抗衰老作用较好,其作用机制可能与正向调控酿酒酵母细胞氧化应激以及能量代谢有关。
OBJECTIVE To screen the best compatibility ratio and administration conditions of Panax ginseng-Poria cocos pair against aging,and investigate its mechanism.METHODS P.ginseng-P.cocos pair extracts with different compatibility ratios(1∶1,1∶2,2∶1,1∶4,4∶1,m/m)were prepared;taking Saccharomyces cerevisiae as the aging model organism,the S.cerevisiae growth curve was drawn by MTT method,the best compatibility ratio,administration concentration and administration time point of P.ginseng-P.cocos pair were screened out;the activities of antioxidant related enzymes[superoxide dismutase(SOD),peroxidase(POD),catalase(CAT)],the levels of reactive oxygen species(ROS)and malondialdehyde(MDA),the content of adenosine triphosphate(ATP),and the mitochondrial membrane potential(MMP)in S.cerevisiae cells were detected;mRNA expressions of SOD1,CTT1,GSH1,ATP1,MRS1 and CDC19 were also detected.RESULTS The optimal ratio of P.ginseng-P.cocos pair for anti-aging activity was 1∶4(m/m),the optimal administration concentration was 220μg/mL,and the optimal administration time point was the 28th hour.The extracts of P.ginseng-P.cocos pair(1∶4,m/m)could significantly increase the activities of SOD,POD and CAT,ATP content,MMP,mRNA expression of CTT1,GSH1 and MRS1(P<0.01),but decrease the levels of MDA and ROS,mRNA expressions of SOD1,ATP1 and CDC19(P<0.05 or P<0.01).CONCLUSIONS P.ginseng-P.cocos pair(1∶4,m/m)has a good anti-aging effect on S.cerevisiae,its mechanism may be related to the positive regulation of oxidative stress and energy metabolism of S.cerevisiae cell.
作者
白竹林
赵大庆
陈静静
曾婧
白雪媛
王思明
BAI Zhulin;ZHAO Daqing;CHEN Jingjing;ZENG Jing;BAI Xueyuan;WANG Siming(Jilin Provincial Academy of Ginseng Science,Changchun University of Chinese Medicine,Changchun 130117,China)
出处
《中国药房》
CAS
北大核心
2022年第21期2584-2589,共6页
China Pharmacy
基金
国家重点研发计划项目(No.2017YFC1702100)。
关键词
人参
茯苓
药对
配伍比例
酿酒酵母
抗衰老
氧化应激
能量代谢
Panax ginseng
Poria cocos
drug pair
compatibility ratio
Saccharomyces cerevisiae
anti-aging
oxidative stress
energy metabolism
