摘要
蛋白质含量是大豆重要的品质性状,受多基因控制,定位大豆蛋白质含量相关位点并挖掘候选基因,对定向培育高蛋白含量大豆品种具有重要意义。本研究以优良品种黑农88作为母本与高蛋白优异种质P73-6B作为父本杂交,构建了一个由265个单株组成的F_(2)群体,利用中豆芯1号对F_(2)群体进行基因型鉴定并构建图谱,结合蛋白质含量表型数据,采用IciMapping 4.2软件在20号染色体上定位了一个QTL,物理距离为2.46 Mb,在区间附近筛选出11个多态性SSR标记并分析群体,将定位区间从2.46 Mb缩小至100.8 kb。增加Gm20_28349696、Gm20_30805913、Gm20_31341532和Gm20_31483719共4个SNP位点,进一步将区间缩小到95.8 kb。对区间内包含的4个基因的9个不同组织在Phytozome v13.1和PPRD RNA-seq 2个数据库中的表达量分析得到了2个候选基因,分别为Glyma.20g081800和Glyma.20g082000基因,本试验结果为大豆蛋白质含量基因克隆及蛋白质调控机制研究提供了理论基础,为大豆高蛋白分子标记育种提供材料和技术支撑。
Protein content is a crucial quality trait of soybean,which is controlled by multiple genes.It is of great significance to locate soybean protein content-related loci and mine candidate genes for directional breeding of soybean varieties with high protein content.In this study,an F2 population consisting of 265 individual plants was constructed by crossing the excellent variety Heinong 88 as the female parent with the high-protein germplasm P73-6B as the male parent.The genotypes of F2 population were identified by using high-density SNP chip of“ZDX1”and the physical map was constructed.Combined with the protein content phenotypic data,the initial mapping interval of a 2.46 Mb QTL was located on chromosome 20 using the IciMapping 4.2 software.Using 11 polymorphic SSR markers screened out,the mapping range was narrowed from 2.46 Mb to 100.8 kb.Adding four SNP markers(Gm20_28349696,Gm20_30805913,Gm20_31341532,and Gm20_31483719),the interval was further reduced to 95.8 kb.The relative expression levels of the four genes contained in the interval in nine different tissues in both data-bases Phytozome v13.1 and PPRD RNA-seq yielded two candidate genes(Glyma.20g081800 and Glyma.20g082000).These re-sults provide a theoretical basis for soybean protein content gene cloning and protein regulation mechanism research,as well as elite material and molecular marker for breeding high protein soybean.
作者
杨硕
武阳春
刘鑫磊
唐晓飞
薛永国
曹旦
王婉
刘亭萱
祁航
栾晓燕
邱丽娟
YANG Shuo;WU Yang-Chun;LIU Xin-Lei;TANG Xiao-Fei;XUE Yong-Guo;CAO Dan;WANG Wan;LIU Ting-Xuan;QI Hang;LUAN Xiao-Yan;QIU Li-Juan(College of Agriculture,Northeast Agricultural University,Harbin 150030,Heilongjiang,China;Heilongjiang Academy of Agricultural Sciences,Harbin 150030,Heilongjiang,China;National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization,Ministry of Agriculture and Rural Affairs/Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China;College of Life Sciences,Jilin Agricultural University,Changchun 130000,Jinlin,China)
出处
《作物学报》
CAS
CSCD
北大核心
2023年第2期310-320,共11页
Acta Agronomica Sinica
基金
国家自然科学基金项目(31960408)
中央级公益性科研院所基本科研业务费专项(S2022ZD02)
中国农业科学院科技创新工程项目资助。
