摘要
目的 为了探索乌拉尔甘草(Glycyrrhiza uralensis Fisch.)TIFY基因家族的特征并预测其功能,本研究在全基因组层面,应用生物信息学方法对乌拉尔甘草TIFY基因家族进行鉴定和分析。方法 在乌拉尔甘草全基因组数据基础上,利用NCBI、MEME及TBtools等工具,结合基因保守结构域,对乌拉尔甘草TIFY基因家族的序列特征、分类、系统发育和基因表达模式进行分析。结果 在乌拉尔甘草中鉴定到TIFY家族基因成员18个(GurTIFY1-GurTIFY18),分别属于TIFY、JAZ、PPD、ZML四个亚家族,编码的核苷酸长度为444-1266 bp,氨基酸长度在147-421 aa之间,等电点为4.57-9.68,分子质量为16741.11-44426.5 Da,含有10个保守基序和4个保守结构域。基因表达结果表明GurTIFY基因中GurTIFY15、GurTIFY4、GurTIFY1、GurTIFY11等在乌拉尔甘草叶和根中的表达水平具有显著差异性。结论 本研究为深入理解TIFY基因响应非生物胁迫的调控机制和参与次生代谢物质累积的功能奠定基础,并为乌拉尔甘草优质品种选育提供科学参考。
Objective To explore the characteristics of the Glycyrrhiza uralensis TIFY gene family and predict its function by using biological information to identify and analyze G. uralensis TIFY gene family from the whole genome level in G. uralensis. Methods Using the existing genomic data of G. uralensis, the GurTIFY genes were identified through bioinformatics analysis tools such as NCBI, MEME and TBtools etc, combined with the gene conserved domain,the sequence characteristics, taxonomy, phylogeny and gene expression patterns of the TIFY gene family were analyzed.Results The study identified 18 members of the TIFY family gene(GurTIFY1-GurTIFY18) in G. uralensis, which belong to four subfamilies: TIFY, JAZ, PPD and ZML. The GurTIFY were composed of 444-1266 bp nucleotides, amino acid length at 147-421 aa, isoelectric points of them were 4.57-9.68 and the molecular mass was 16741.11-44426.5Da. By analyzing the gene structure, conservative motif could be known that the GurTIFY gene family contains 10conservative motifs and 4 conservative domains. In addition, this study also analyzed the expression model of TIFY gene family members. The results showed that GurTIFY15, GurTIFY4, GurTIFY1, GurTIFY11 had significant differences in the leaf and roots of G. uralensis in all GurTIFY.Conclusion This study comprehensively analyzes the TIFY family in G.uralensis. The results of the study have laid the foundation for the in-depth understanding of the regulatory mechanism of TIFY gene response non-biodarity and the formation of the accumulation of secondary metabolism. This study will provide valuable reference for gene function study of the TIFY family genes in G. uralensis and G. uralensis breeding.
作者
秦振芬
孟祥霄
温东
朱雪雯
王艳
Botir Khaitov
Atia tul Wahab
孙伟
Qin Zhenfen;Meng Xiangxiao;Wen Dong;Zhu Xuewen;Wang Yan;Botir Khaitov;Atia tul Wahab;Sun Wei(Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193,China;H.E.J.Research Institute of Chemistry,International Center for Chemical and Biological Sciences,University of Karachi,Karachi 75270,Pakistan;International Center for Biosaline Agriculture,Tashkent 100084,Uzbekistan;Dr.Panjwani Center for Molecular Medicine and Drug Research,International Center for Chemical and Biological Sciences,University of Karachi,Karachi 75270,Pakistan)
出处
《世界科学技术-中医药现代化》
CSCD
北大核心
2022年第5期1855-1864,共10页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家科学技术部国家重点研发计划(2018YFC1706500):甘草全产业链技术体系升级与产品开发,负责人:边育红
国家科学技术部国家重点研发计划(2021YFE0100900):巴基斯坦传统药物资源开发与保护,负责人:宋驰
中国中医科学院科技创新工程(CI2021A03710):基于各国药典的全球草药数据库的构建,负责人:宋驰。
关键词
生物信息学
乌拉尔甘草
TIFY基因家族
鉴定
功能分析
Bioinformatics
Glycyrrhiza uralensis Fisch
TIFY gene family
Identify
Functional analysis
