摘要
目的 研究金沸草的化学成分及其对HaCat细胞的影响。方法 金沸草提取物采用硅胶、ODS、Sephadex LH-20柱色谱进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。结果 从中得到15个化合物,分别鉴定为mutabiloside(1)、6-羟基木犀草素(2)、木犀草素-7, 3′, 4′-三甲醚(3)、5, 4′-二羟基-6, 7, 3′, 5′-四甲氧基黄酮(4)、5, 4′-二羟基-7, 3′-二甲氧基黄酮(5)、5-羟基-7, 2′, 4′, 5′-四甲氧基黄酮(6)、2, 4-二羟基-6-甲基苯乙酮(7)、对羟基肉桂酸甲酯(8)、对羟基肉桂酸乙酯(9)、对羟基苯甲酸乙酯(10)、diptoindonesin D(11)、邻苯二甲酸二乙酯(12)、7-O-feruloylorientin(13)、pinoresinol(14)、moscatin(15)。化合物1、11、13、15对HaCat细胞具有不同程度的抑制作用。结论 所有化合物均为首次从金沸草中分离得到,金沸草能一定程度上抑制HaCat细胞的增殖。
AIM To study the chemical constituents from Inula japonica Thunb. and their effects on HaCat cells.METHODS The extract from I.japonica was isolated and purified by silica gel, ODS, Sephadex LH-20 column chromatography, then the structures of obtained compounds were identified by physicochemical properties and spectral data.RESULTS Fifteen compounds were isolated and identified as mutabiloside(1), 6-hydroxyluteolin(2), luteolin-7, 3′, 4′-trimethyl ether(3), 5, 4′-dihydroxy-6, 7, 3′, 5′-tetramethoxy flavonoids(4), 5, 4′-dihydroxyl flavonoids(5), 5-hydroxy-7, 2′, 4′, 5′-tetramethoxy flavone(6), 2, 4-dihydroxy-6-methylaceto-phenone(7), methyl p-hydroxycinnamate(8), ethyl p-hydroxycinnamate(9), ethyl p-hydroxybenzoate(10), diptoindonesin D(11), diethyl phthalate(12), 7-O-feruloylorientin(13), pinoresinol(14), moscatin(15).Compands 1,11,13 and 15 had different inhibitory effects on HaCat cells.CONCLUSION All the compounds are isolated from I.japonica for the first time. I.japonica can inhibit proliferation of HaCat cells to a certain extent.
作者
董明亮
马新苹
郭金兰
甘才斌
张步鑫
DONG Ming-liang;MA Xin-ping;GUO Jin-lan;GAN Cai-bin;ZHANG Bu-xin(Xinxiang Central Hospital,The Fourth Clinical College Xinxiang Medical University,Xinxiang 453000,China;Henan Province Hospital of Traditional Chinese Medicine,the Second Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou 450002,China)
出处
《中成药》
CAS
CSCD
北大核心
2022年第11期3526-3530,共5页
Chinese Traditional Patent Medicine
基金
河南省中医药科学研究专项课题(2019JDZX099)。
关键词
金沸草
化学成分
分离鉴定
HACAT细胞
Inula japonica Thunb.
chemical constituents
isolation and identification
