摘要
目的建立当归Angelicae sinensis的HPLC指纹图谱并测定其主要有效成分的含量,结合多种化学计量学分析,确定不同产地不同品种(岷归1号、岷归2号)当归差异成分,为当归药材质量控制提供参考。方法采用HPLC法建立不同品种当归的指纹图谱,并对指标成分进行含量测定。采用相似度评价、聚类分析(cluster analysis,CA)、因子分析、主成分分析(principal component analysis,PCA)、正交偏最小二乘法判别分析(orthogonal partial least squares discriminant analysis,OPLS-DA)、Fisher线性判别分析进行数据分析。结果HPLC指纹图谱共标定了不同品种当归中的16个共有峰,指认出绿原酸、阿魏酸、洋川芎内酯I、洋川芎内酯H、洋川芎内酯A、阿魏酸松柏酯、藁本内酯、丁烯基苯酞8个色谱峰。岷县产区当归的2个品种当归间存在差异的成分是绿原酸(P<0.01)、洋川芎内酯I(P<0.01)、洋川芎内酯H(P<0.01)、藁本内酯(P<0.01)和丁烯基苯酞(P<0.05),渭源产区的是洋川芎内酯I(P<0.01)、洋川芎内酯H(P<0.01)、洋川芎内酯A(P<0.01)、藁本内酯(P<0.01);临洮产区的是绿原酸(P<0.01)、阿魏酸(P<0.01)、阿魏酸松柏酯(P<0.01)、丁烯基苯酞(P<0.05);通渭产区的是阿魏酸(P<0.01)、洋川芎内酯H(P<0.05)、洋川芎内酯A(P<0.05)、藁本内酯(P<0.01)。结论建立的HPLC指纹图谱结合含量测定、CA、因子分析、PCA、OPLS-DA分析可以客观、全面、有效地确定不同产地不同品种当归中主要有效成分的差异,可为当归品种鉴别及质量控制提供有力支撑。
Objective The HPLC fingerprint of Angelicae sinensis was established and the content of its main effective components was determined.Combined with a variety of stoichiometric analyses,the different components of different varieties(Mingui Ⅰ and Mingui Ⅱ)of A.sinensis were determined to provide a reference for its quality control.Methods HPLC method was used to establish the fingerprint of different varieties of A.sinensis and determine the content of index components.The data were analyzed by similarity evaluation combined with cluster analysis(CA),factor analysis,principal component analysis(PCA),orthogonal partial least squares discriminant analysis(OPLS-DA),Fisher linear discriminant(FLDA).Results A total of 16 common peaks in A.sinensis from different origins were calibrated by HPLC fingerprint.Eight chromatographic peaks of chlorogenic acid,ferulic acid,senkyunolide I,senkyunolide H,senkyunolide A,coniferyl ferulate,ligustilide,and butenyl phthalide were identified.The differences between the two varieties of A.sinensis in the Min County were chlorogenic acid(P<0.01),senkyunolide I(P<0.01),senkyunolide H(P<0.01),ligustilide(P<0.01))and butenyl phthalide(P<0.01),in Weiyuan,were senkyunolide I(P<0.01),senkyunolide H(P<0.01),senkyunolide A(P<0.01),ligustolide(P<0.01);In Lintao werechlorogenic acid(P<0.01),ferulic acid(P<0.01),coniferyl ferulate(P<0.01),butenyl phthalide(P<0.05);In Tongwei were ferulic acid(P<0.01),senkyunolide H(P<0.05),senkyunolide A(P<0.05),and ligustolide(P<0.01).Conclusion The established HPLC fingerprint combined with content determination,CA,factor analysis,PCA,OPLS-DA can objectively,comprehensively,and effectively determine the differences of main effective components in different varieties of A.sinensis,and it can provide strong support for the variety identification and quality control of A.sinensis.
作者
张明惠
朱田田
晋玲
王富胜
徐丽
康舒淇
ZHANG Ming-hui;ZHU Tian-tian;JIN Ling;WANG Fu-sheng;XU Li;KANG Shu-qi(Gansu University of Traditional Chinese Medicine,Lanzhou 730000,China;Northwest Collaborative Innovation Center for Traditional Chinese Medicine Co-constructed by Gansu Province&MOE of PRC,Lanzhou 730000,China;Engineering Research Center for Evaluation,Protection,and Utilization of Rare Traditional Chinese Medicine Resources,Gansu Province,Lanzhou 730000,China;Dingxi Academy of Agricultural Sciences,Dingxi 743000,China)
出处
《中草药》
CAS
CSCD
北大核心
2022年第19期6187-6199,共13页
Chinese Traditional and Herbal Drugs
基金
甘肃省科技厅创新基地和人才计划目(20JR5RA182)
道地药材生态种植及质量保障项目项目(国中医药科技[2020]153号)
甘肃省教育厅“双一流”科研重点项目(GSSYLXM-05)
甘肃中医药大学科学研究与创新基金项目(2021KCZD-4)
西北中藏药省部共建协同创新中心开放基金资助项目(Xbzzy202207)。
关键词
当归
品种差异
绿原酸
阿魏酸
洋川芎内酯I
洋川芎内酯H
洋川芎内酯A
阿魏酸松柏酯
藁本内酯
丁烯基苯酞
HPLC指纹图谱
化学模式识别
Angelicae sinensis(Oliv.)Diels
variety differences
HPLC fingerprint
chlorogenic acid
ferulic acid
senkyunolide I
senkyunolide H
senkyunolide A
coniferyl ferulate
ligustilide
butenyl phthalide
chemical pattern recognition
