摘要
C_(6)型转录因子在调控植物病原真菌生长发育及致病过程中起重要的作用。从胶孢炭疽菌中鉴定了1个C_(6)型转录因子Cgctf1,该基因编码405个氨基酸,含有1个GAL4结构域。利用同源重组的方法获得了Cgctf1基因的敲除突变体,并在突变体上的基础上获得互补株。表型分析发现,敲除突变体在营养生长方面同野生型相比无明显差异,但突变体对盐胁迫较为敏感,对H_(2)O_(2)和SDS的抗性增强;敲除突变体分生孢子产量较野生型减少,孢子萌发率和附着胞形成率均明显降低;洋葱表皮侵染及玻璃纸侵入试验均表明敲除突变体分生孢子侵入能力减弱;在橡胶叶片上,敲除突变体形成的病斑小于野生型,致病力减弱。结果表明,Cgctf1参与调控胶孢炭疽菌的分生孢子发育、附着胞形成及侵入、胁迫响应和致病性。
C_(6)-type transcription factor plays an important role in regulating the growth,development and pathogenicity of plant pathogenic fungi.A C_(6)-type transcription factor geneCgctf1 was identified from C.gloeosporioides,which encodes 405 amino acids and contains a GAL4 domain.Gene-knockout mutants ofCgctf1 were obtained by homologous recombination,and the complementary strain was obtained on the basis of the mutants.Phenotypic analysis showed that there was no significant difference in vegetative growth between the knockout mutant and the wild type,but the mutant was more sensitive to salt stress and more resistant to H_(2)O_(2) and SDS.The conidia yield,spore germination rate and appressorium formation rate of the knockout mutant were significantly lower than those of the wild type.Onion epidermis infection and cellophane invasion tests showed that the penetration ability of conidia of the knockout mutant was weakened.On the rubber leaves,the disease spot formed by knockout mutants was smaller than that of wild type,and the mutants had reduced virulence.These results suggest thatCgctf1 is involved in the regulation of conidial development,appressorium formation and invasion,stress response and pathogenicity of C.gloeosporioides.
作者
曹健
刘沙玉
王地广
柳志强
CAO Jian;LIU Shayu;WANG Diguang;LIU Zhiqiang(School of life sciences,Hainan University,Haikou 570228,China)
出处
《生物学杂志》
CAS
CSCD
北大核心
2022年第6期41-46,共6页
Journal of Biology
基金
海南省自然科学基金项目(319MS011)。
关键词
橡胶炭疽病
胶孢炭疽菌
C_(6)型转录因子
分生孢子
致病性
Rubber anthracnose
Colletotrichum gloeosporioides
C_(6)-type transcription factor
conidium
pathogenicity
