摘要
目的探讨原花青素对过氧化脲漂白后牙本质与复合树脂粘接强度的影响。方法按照牙本质粘接界面不同处理方法,将120颗人离体第三磨牙随机分为12组(n=10);W组(无漂白+去离子水)、Wa组(无漂白+去离子水+老化)、WT1组(无漂白+5%原花青素1 min)、WT1a组(无漂白+5%原花青素1 min+老化)、WT2组(无漂白+5%原花青素5 min)、WT2a组(无漂白+5%原花青素5 min+老化)、C组(过氧化脲+去离子水)、Ca组(过氧化脲+去离子水+老化)、CT1组(过氧化脲+5%原花青素1 min)、CT1a组(过氧化脲+5%原花青素1 min+老化)、CT2组(过氧化脲+5%原花青素5 min)、CT2a组(过氧化脲+5%原花青素5 min+老化);上述各组在即刻或冷热循环老化后,万能力学试验机测试粘接强度,扫描电镜观察粘接界面微观形貌和纳米渗漏。结果CT1组经原花青素处理1 min(P<0.001)和CT2组经原花青素处理5 min(P<0.001)的漂白即刻组粘接强度均高于对照组C组,差异具有统计学意义,但CT1与CT2之间差异无统计学意义(P=1.000);冷热循环处理后各组粘接强度均下降,W与Wa(P<0.001)、C与Ca(P<0.001)的差异具有统计学意义,但CT1与CT1a(P=0.052)、CT2与CT2a(P=0.053)之间的差异无统计学意义。“漂白”(P<0.001)、“老化”(P<0.001)、“原花青素”(P<0.001)主效应和二阶交互效应“漂白*原花青素”(P=0.008)、“漂白*老化”(P=0.024)、“老化*原花青素”(P<0.001)均有统计学意义。扫描电镜示即刻组C、CT1和CT2组混合层欠清晰,老化组Ca、CT1a和CT2a组混合层局部破坏崩解,背散射模式下Ca组混合层存在大量硝酸银颗粒,CT1a、CT2a组混合层残留银离子减少。结论5%原花青素预处理1 min即可提高过氧化脲漂白后牙本质与复合树脂的即刻粘接强度并改善粘接耐久性。
Objective To study the influence of procyanidins on the bonding strength of dentin bleached by carbamide peroxide to composite resin. Methods By applying different treatments to dentin bonding interfaces, 120 human third molars were randomly divided into 12 groups(n = 10): W group(no bleaching+deionized water), Wa group(no bleaching + deionized water + aging), WT1 group(no bleaching + 5% procyanidins for 1 min), WT1a group(no bleaching+5% procyanidins for 1 min+aging), WT2 group(no bleaching+5% procyanidins for 5 min), WT2a group(no bleaching+5% procyanidins for 5 min+aging), C group(carbamide peroxide+deionized water), Ca group(carbamide peroxide+deionized water+aging), CT1 group(carbamide peroxide+5% procyanidins for 1 min), CT1a group(carbamide peroxide+5% procyanidins for 1 min+aging), CT2 group(carbamide peroxide+5% procyanidins for 5 min), and CT2a group(carbamide peroxide+5% procyanidins for 5 min+aging). The bond strength to composite resin was measured by universal mechanical testing machine, microstructure and the nanoleakages were measured by scanning electron microscope immediately or after the thermal cycling aging test. Results The immediate bond strength of the bleached groups pretreated with procyanidins for 1 min(P<0.001) and 5 min(P<0.001) was higher than that of Group C, and the difference was statistically significant. Meanwhile, there was no statistically significant difference between Group CT1 and Group CT2(P = 1.000). After the thermal cycles, the bond strength of each group declined. The differences between Group W and Group Wa(P<0.001) and Group C and Group Ca(P<0.001) were statistically significant, but no significant differences between Group CT1 and Group CT1a(P = 0.052) or Group CT2 and Group CT2a(P = 0.053)were found. The main effects of“aging”(P<0.001),“bleaching”(P<0.001) and “procyanidins”(P<0.001) and the second-order interaction effects of “bleachingprocyanidins”(P = 0.008),“bleachingaging”(P = 0.024), and “agingprocyanidins”(P<0.001) were statistically significant. SEM observations showed that the hybrid layers in Groups C, CT1 and CT2 were not clear, and the hybrid layers in Groups Ca, CT1a and CT2a were partially destroyed and disintegrated. Under backscattering mode, it was observed that there were a large number of silver nitrate particles in the hybrid layer of Group Ca, and the residual silver ions in the hybrid layer of Groups CT1a and CT2a were decreased. Conclusion Pretreatment with 5% procyanidins for 1 min can improve the immediate bond strength of dentin bleached by carbamide peroxide to composite resin and maintain bonding durability.
作者
李姝宏
陈虹西
周利平
杨倩
谢翠柳
LI Shuhong;CHEN Hongxi;ZHOU Liping;YANG Qian;XIE Cuiliu(School of Stomatology of Southwest Medical University,Luzhou 646000,China;Department of Prosthodontics,the Affiliated Stomatology Hospital of Southwest Medical Uni-versity,Luzhou 646000,China)
出处
《口腔疾病防治》
2023年第4期245-251,共7页
Journal of Prevention and Treatment for Stomatological Diseases
基金
四川省大学生创新创业训练计划(S202010632267)。
关键词
原花青素
牙齿漂白
过氧化脲
牙本质粘接
复合树脂
粘接强度
微观形貌
纳米渗漏
粘接耐久性
procyanidins
tooth⁃bleaching
carbamide peroxide
dentin adhesive
composite resin
bond strength
microstructure
nanoleakage
bonding durability
