摘要
[目的]建立4种双香豆素类化合物(biscoumarin,BCM)的液-质分析方法;评价4种BCM对斑马鱼的安全性。[方法]采用液-质联用仪测定水中4种双香豆素类化合物的含量,确定其暴露浓度;采用半静态法测定双香豆素类天然产物衍生物对斑马鱼的急性毒性。[结果]BCM4、BCM7、BCM8、BCM12标样的峰面积(y)与质量浓度(x)呈线性关系,决定系数R^(2)均>0.9900,最低检测浓度LOQ为0.2 mg/L,BCM4、BCM8、BCM12高、低质量浓度的添加剂量分别为80.0、20.0 mg/L,BCM7高、低质量浓度的添加剂量分别为8.0、2.0 mg/L,4种BCM的添加回收率介于95.20%~113.60%之间。BCM4、BCM7、BCM8、BCM12对斑马鱼急性毒性96 h的LC分别为33.75、13.31、41.19、48.42 mg/L。[结论]使用高效液-质联用仪测定BCM4、BCM7、BCM8、BCM12在水中的含量所采用的检测方法精密度和准确度良好,4种BCM对斑马鱼的急性毒性为低毒。
[Aims] This study aims to establish a LC-MS analysis method for evaluation of the safety of 4 biscoumarin compounds BCM on zebrafish. [Methods] The contents of four biscoumarin compounds in water were texted by LC-MS and their exposure concentrations were determined. The acute toxicity of biscoumarin derivatives to zebrafish was determined by semi-static method. [Results] The peak area(y) of BCM4, BCM7, BCM8 and BCM12 samples showed a linear relationship with the concentration(x), and the determination coefficients R^(2)were all >0.9900, the lowest detection concentration LOQ was 0.2 mg/L, the high and low concentrations of BCM4, BCM8, BCM12 were80.0, 20.0 mg/L, the high and low concentrations of BCM7 were 8.0, 2.0 mg/L, respectively. The recoveries of the four kinds of BCM ranged from 95.20% to 113.60%. The LCof acute toxicity of BCM4, BCM7, BCM8 and BCM12 to zebrafish at 96 h were 33.75, 13.31, 41.19 and 48.42 mg/L, respectively. [Conclusions] The determination method of BCM4, BCM7, BCM8 and BCM12 in water by LC-MS was precise and accurate, and the acute toxicity of four kinds of BCM to zebrafish was low.
作者
范继文
李维政
梁赫
赵娜娜
王天琴
张乐
路伟
FAN Ji-wen;LI Wei-zheng;LIANG He;ZHAO Na-na;WANG Tian-qin;ZHANG Le;LU Wei(Engineering Research Centre of Cotton,Ministry of Education,College of Agriculture,Xinjiang Agricultural University,Urumqi 830052,China;Guorino Drug Safety Evaluation Research Co.,Ltd.,Xi'an 710000,China)
出处
《农药》
CAS
CSCD
北大核心
2022年第11期827-832,共6页
Agrochemicals
基金
国家重点研发计划项目(2017YFD0201903)
天山创新团队计划项目(2020D14001)
新疆农科院科技创新重点培育专项(xjkcpy-2020004)。
