摘要
在四川省雅安市雨城区雷竹林区丛枝病发生普遍且危害严重。为明确该病的发生规律和病原种类,以及病原的生物学特性和野外药剂防效,本研究对6个雷竹丛枝病发病林区进行调查与样品采集,通过野外观测与分析,掌握了该病害发生特点和演变规律;运用表型特征观测与多基因(ITS、LSU、SSU、tef1-α、rpb2、mcm7和tub2)系统发育分析,明确该病害病原为竹针孢麦角菌Aciculosporium take;采用不同培养基、pH、光照、碳氮源条件培养病原菌,其最适生长和产孢的培养基为PDA,最佳pH值为7.5,最佳碳源为葡萄糖,最佳氮源为牛肉膏+酵母膏,最佳光照为24 h全光照;依据含药平板试验,10%苯醚甲环唑WG抑菌效果最佳,EC50值为0.019μg/mL,野外防治试验中,浓度为400μg/mL的苯醚甲环唑,防治效果达96.57%。
The witches’broom disease widespread in Yucheng District of Ya’an city in Sichuan Province has caused serious harm to Phyllostachys violascens.To clarify the occurring rules of this disease and biological character of the pathogen,an investigation was conducted in six bamboo forests in Ya’an city,and diseased samples were collected.The pathogen was identified as Aciculosporium take on the basis of combining morphology observation and multi-gene phylogenetic analyses of a combined seven-gene dataset(ITS,LSU,SSU,tef1-α,rpb2,mcm7 and tub2).The culture experiments under different conditions revealed that the optimum medium for growth and sporulation of the pathogen was PDA at pH value of 7.5.Carbon source was glucose,and nitrogen source was beef extract and yeast extract,and 24-hour light was optimal for growth.Fungicide plate tests showed that 10%difenoconazole(WG)gained the best control effect(EC50=0.019μg/mL).In the field test,the control efficiency based on disease index was 96.57%,adopting difenoconazole(WG)with a concentration of 400μg/mL.
作者
刘思怡
杨春琳
曾倩
吕一聪
许秀兰
刘利娟
刘应高
LIU Siyi;YANG Chunlin;ZENG Qian;LV Yicong;XU Xiulan;LIU Lijuan;LIU Yinggao(College of Forestry,Sichuan Agricultural University,Chengdu 611130,Sichuan,China;Research Institute of Forestry,Chengdu Academy of Agricultural and Forestry Sciences,Chengdu 611130,Sichuan,China)
出处
《菌物学报》
CAS
CSCD
北大核心
2022年第11期1867-1888,共22页
Mycosystema
关键词
竹针孢麦角菌
发生规律
杀菌剂
防效试验
Aciculosporium take
occurring rules
fungicide
control effect experiment
