摘要
为了揭示黄腐酸对干旱胁迫下燕麦光合及其抗氧化酶活性的影响机理,该研究选用燕麦品种‘燕科2号’为材料,采用盆栽试验,以正常供水(田间持水量的75%)为对照(CK),设干旱胁迫处理(田间持水量的45%,D0)、D0+喷施不同浓度黄腐酸(0、200、400、600、800、1000 mg/L)处理(D1~D5),测定各处理燕麦干鲜重、光合性能及其抗氧化酶活性。结果表明:(1)与CK相比,干旱胁迫下燕麦幼苗地上部鲜重和干重、叶片光合色素含量、净光合速率(P_(n))、蒸腾速率(T_(r))、气孔导度(G_(s))均显著降低,并导致叶片F_(v)/F_(m)、q P、ETR和ΦPSⅡ显著下降,使叶片抗氧化酶SOD、POD、CAT活性分别显著提高25.68%、19.98%和7.29%。(2)与D0相比,D0+喷施600 mg/L黄腐酸后,燕麦幼苗地上部鲜重和干重分别显著提高了28.59%和39.13%,叶片叶绿素a、叶绿素a+b、类胡萝卜素和P_(n)、G_(s)、T_(r)及F_(v)/F_(m)、ΦPSⅡ、ETR分别显著增加了25.17%、21.03%、47.37%和74.38%、26.47%、43.34%及6.49%、69.57%、70.71%,C_(i)和F_(o)、NPQ分别显著降低了19.52%和13.32%、43.75%。(3)干旱胁迫下喷施不同浓度的黄腐酸均使幼苗叶片中SOD、POD、CAT活性较D0处理显著增加,其中喷施600 mg/L黄腐酸的叶片SOD、POD、CAT活性最高,分别较D0处理显著增加了12.19%、76.57%和55.26%。研究认为,叶面喷施适宜浓度黄腐酸能够显著提高干旱胁迫下燕麦幼苗的光合作用及其抗氧化能力,缓解干旱对燕麦幼苗的伤害,进而促进燕麦幼苗的生长,且以叶面喷施600 mg/L黄腐酸效果最佳。
In order to reveal the influence mechanism of fulvic acid on the photosynthesis and antioxidant enzyme activity of Avena sativa under drought stress,this study selected A.sativa variety‘Yanke 2’as the material,adopted pot experiment,took normal water supply(75%of field capacity)as the control(CK),set drought stress treatment(45%of field capacity,D0),D0 and spraying fulvic acid of different concentrations(0,200,400,600,800,1000 mg/L)treatment(D1-D5),The dry and fresh weight,photosynthetic performance and antioxidant enzyme activity of A.sativa were measured.The results showed that:(1)compared with CK,under drought stress,the aboveground fresh and dry weight,leaf photosynthetic pigment content,net photosynthetic rate(P_(n)),transpiration rate(T_(r)),stomatal conductance(G_(s))of A.sativa seedlings were significantly reduced,and F_(v)/F_(m),q P,ETR andΦPSⅡdecreased significantly.The activities of SOD,POD and CAT in leaves were increased by 25.68%,19.98%and 7.29%respectively.(2)Compared with D0,after spraying 600 mg/L fulvic acid,the shoot fresh weight and dry weight of A.sativa seedlings were significantly increased by 28.59%and 39.13%,respectively.Chlorophyll a,chlorophyll a+b,carotenoids content and P_(n),G_(s),T_(r),F_(v)/F_(m),ΦPSⅡand ETR increased by 25.17%,21.03%,47.37%and 74.38%,26.47%,43.34%and 6.49%,69.57%and 70.71%,respectively,and C_(i),F_(o)and NPQ decreased by 19.52%,13.32%and 43.75%,respectively.(3)Under drought stress,the activities of SOD,POD and CAT in leaves of seedlings sprayed with fulvic acid of different concentrations were significantly increased compared with D0 treatment.Among them,the activities of SOD,POD and CAT in leaves sprayed with fulvic acid of 600 mg/L were the highest,which were significantly increased by 12.19%,76.57%and 55.26%compared with D0 treatment.The study shows that foliar spraying suitable concentration of fulvic acid can improve the photosynthesis and antioxidant capacity of A.sativa seedlings under drought stress,alleviate the damage of drought to A.sativa seedlings,and then promote the growth of A.sativa seedlings,and the effect of foliar spraying of 600 mg/L fulvic acid is the best.
作者
朱珊珊
米俊珍
赵宝平
武俊英
王莹
刘景辉
ZHU Shanshan;MI Junzhen;ZHAO Baoping;WU Junying;WANG Ying;LIU Jinghui(College of Agriculture,Inner Mongolia Agricultural University,Hohhot 010019,China)
出处
《西北植物学报》
CAS
CSCD
北大核心
2022年第11期1902-1909,共8页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金(32160508)
国家燕麦荞麦产业技术体系(CARS-08-B-5)。
关键词
燕麦
干旱胁迫
黄腐酸
光合参数
抗氧化酶
Avena sativa
drought stress
fulvic acid
photosynthetic parameters
antioxidant enzyme
