摘要
研究植物性状及与植物密切相关的微生物群落沿海拔的变异,有助于理解个体对环境变化的适应能力。以生存于不同海拔高度的极小种群野生植物百山祖冷杉(Abies beshanzuensis)成年个体(海拔951、1400和1590 m处为人工培育实生苗,海拔1750m处为野生成年个体)为研究对象,利用根系扫描仪测定百山祖冷杉细根性状,利用高通量测序技术分析百山祖冷杉根际土壤及根内微生物(细菌及真菌)群落组成,探究根际土壤及根内微生物群落和细根性状在不同海拔高度的差异。结果表明:不同海拔高度的百山祖冷杉周围土壤养分有效性较差,理化性质除铵态氮含量和pH值外均存在显著差异(P<0.05)。根际土壤及根内真菌和细菌群落沿海拔变异明显。为适应土壤生物及非生物因子沿海拔的变化,百山祖冷杉采取不同的细根营养策略,细根具有高度可塑性,与外生菌根真菌产生协同效应缓解了百山祖冷杉受到的生存限制。
Studies on the variation of plant traits and plant-associated microbiomes at different altitudes will contribute to understanding the adaptation of plants to climate change. In this study, we selected three extant adults of A. beshanzuensis at 1 750 m and other adults from artificially cultivated seedlings at different altitudes(951, 1 400, and 1 590 m), and investigated their differences in root traits and microbial community compositions in the rhizosphere soil and roots. We used a root scanner and high-throughput sequencing to explore these differences at different altitudes. The main results were as follows: the nutrient availability around A. beshanzuensis was low. There were significant differences in physicochemical properties at different altitudes, except in ammonium nitrogen and pH value(P<0.05). The fungal and bacterial communities in the rhizosphere soil and roots at different altitudes have obvious variance. In order to adapt to the variation in soil biotic and abiotic factors across altitudes, A. beshanzuensis adopted different fine root foraging strategies. The fine roots of A. beshanzuensis showed high plasticity and could effectively cooperate with ECM fungi to increase fitness.
作者
胡瑞
吴宪
吴友贵
刘宇
HU Rui;WU Xian;WU Yougui;LIU Yu(School of Ecology and Environmental Science,East China Normal University,Shanghai200241,China;Management of Baishanzu,Fengyangshan-Baishanzu National Nature Reserve,Lishui,Zhejiang323808,China;Shanghai Institute of Pollution Control and Ecological Security,Shanghai200092,China)
出处
《森林与环境学报》
CSCD
北大核心
2023年第1期84-91,共8页
Journal of Forest and Environment
基金
浙江凤阳山-百山祖国家级自然保护区科研基金项目(2020FWR0208)
国家自然科学基金项目“全球变暖对土壤病原真菌维持森林树种多样性纬度格局的影响”(31670531)、“土壤病原真菌在亚热带森林稀有树种维持方面的作用研究”(32071645)。
关键词
百山祖冷杉
海拔梯度
细根性状
微生物群落组成
植物-微生物相互作用
Abies beshanzuensis
altitudinal gradient
fine root traits
microbial community composition
plant-microbiome interactions
