摘要
为了解我国副猪嗜血杆菌(HPS)和猪链球菌(SS)血清型的流行趋势,本研究利用PCR对2020年~2021年国内分离的52株HPS和69株SS分别检测二者的血清型;通过查阅2014年~2020年国内外文献报道的402株HPS和189株SS的血清型,并与上述分离的HPS、SS的血清型作比较,分析二者血清型的流行趋势。结果显示,2020年~2021年HPS主要优势血清型占比由高到低依次为12>4=5>13型,SS主要优势血清型占比由高到低依次为2或1/2>5>不确定型(US)>8=18>27>15型;文献检索结果显示:2014年~2020年HPS主要优势血清型占比从高到低依次为4>5>US>13>14>7型,SS主要优势血清型为2或1/2>7>9>US。表明血清4、5、13型仍是HPS的优势血清型,且12型升高了28%;血清2或1/2仍然是我国SS的优势血清型,且5、15、18、27型明显增加,7和9型减少,US型仍然较高。采用K-B法检测HPS与SS分离株的耐药性,采用PCR检测二者的耐药基因(7大类共27种耐药基因),并分析二者的耐药基因与耐药表型的相关性。药敏试验结果显示,两类分离菌中对β-内酰胺类、大环内酯类和磺胺类药物耐药的菌株高达88.31%(68/77),对氨基糖苷类和四环素类药物耐药的菌株分别约为80.52%(62/77)和76.62%(59/77),对酰胺醇类和喹诺酮类药物耐药的菌株较少。且二者均存在严重的多重耐药性,其中双重耐药的菌株占比最高为28.57%(22/77),4重、5重、6重及8重耐药的菌株均为14.29%(11/77)。耐药基因检测结果显示,两类细菌均检出了β-内酰胺类、氨基糖苷类、磺胺类和Ⅰ类整合子4大类耐药基因,特别是β-内酰胺类的bla-TEM、磺胺类的sulI耐药基因检出率均为100%。其中100%HPS分离株检出氨基糖苷类的Aph(3’)-Ia、Ⅰ类整合子的3’CS和Int1耐药基因;100%SS分离株检出Ⅰ类整合子的VR耐药基因。两株菌耐药基因与耐药表型相关性分析结果显示,β-内酰胺类和磺胺类耐药表型与耐药基因之间的符合率达88%;氨基糖苷类耐药表型与耐药基因之间的符合率为72%;四环素类和喹诺酮类耐药基因和耐药表型之间不符合。上述结果表明,HPS和SS分离株的耐药性均较强,且其部分耐药表型与其携带的耐药基因相关性较强。本研究为规模化养殖场HPS和SS感染的临床用药及疫苗研发提供了数据支持,并为后续相关疾病的防控奠定了基础。
To investigate the epidemic trend of serotypes of Haemophilus parasuis(HPS)and Streptococcus suis(SS)in China,PCR was applied to test the serotypes of 52 strains of HPS and 69 strains of SS isolated in China during 2020 to 2021.The information on serotypes of 402 strains of HPS and 189 strains of SS reported in domestic and abroad during 2014 to 2020 were searched,and the results were compared with the above tested serotypes of HPS and SS to analyze the epidemiological trends.The results showed that during 2020 to 2021,the dominant serotypes of HPS from high to low were 12>4=5>13,and the dominant serotypes of SS were types 2 or 1/2>5>undetermined serotype(US)>8=18>27>15.The results of literature search showed that the dominant serotypes of HPS during 2014 to 2020 were types 4>5>US>13>14>7,the dominant serotypes of SS were types 2 or 1/2>7>9>US.The above results indicate that serotypes 4,5 and 13 were still the dominant serotypes of HPS,particularly the proportion of type 12 was increased by 28%.Serotype 2 or 1/2 is still the dominant serotype of SS in China,with significant increases in serotypes 5,15,18 and 27,and decreases in serotypes 7 and 9.The proportion of undetermined serotypes is still high.Drug resistant phenotypes of the HPS and SS isolates were detected by K-B method,27 drug resistant genes were detected by PCR,and the coincidence rates between drug resistant genes and phenotypes were analyzed.The results of antimicrobial susceptibility test showed that 88.31%(68/77)isolates were resistant toβ-lactams,macrolides,and sulfonamides,80.52%(62/77)and 76.62%(59/77)isolates were resistant to aminoglycosides and tetracyclines,respectively,and fewer strains were resistant to amide alcohols and quinolones.Moreover,multidrug resistance of HPS and SS was severe,and the highest proportion of dual drug resistant strains was28.57%(22/77),and the strains with quadruple,quintuple,sextuple and octuple drug resistance were all 14.29%(11/77).The detection of resistant genes showed that the resistant genes ofβ-lactams,aminoglycosides,sulfonamides and typeⅠintegrons were confirmed in the isolates of HPS and SS,the detection rates were up to 100%for bla-TEMofβ-lactam and SulI of sulfonamides.All HPS isolates carried the resistant genes of aminoglycoside Aph(3’)-Ia,typeⅠintegron 3’CS and Int1,and all SS isolates carried the resistant genes of typeⅠintegrons VR.The analysis of correlation between drug resistant genes and phenotypes showed that the coincidence rate ofβ-lactam and sulfonamides was all 88%,and it was 72%for aminoglycosides.There was no match between drug resistant phenotypes and genotypes of tetracyclines and quinolones.These results indicate the severe drug resistance of HPS and SS isolates and strong relation between some drug resistant phenotypes and genotypes.This study provides data support for development of clinical drug and vaccine against HPS and SS in large-scale farms and lays a foundation for subsequent prevention and control of the related diseases.
作者
董婉玉
王馨雨
张聚民
王晴雨
肖敏
任华
卢昌
周莹珊
杜静
于浩然
仲伟
宋厚辉
王晓杜
DONG Wan-yu;WANG Xin-yu;ZHANG Ju-min;WANG Qing-yu;XIAO Min;REN Hua;LU Chang;ZHOU Ying-shan;DU Jing;YU Hao-ran;ZHONG Wei;SONG Hou-hui;WANG Xiao-du(Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province,Zhejiang Provincial Research Center for Animal Health Diagnostics&Advanced Technology,Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management,China-Australia Joint Laboratory for Animal Health Big Data Analytics,College of Animal Science and Technology&College of Veterinary Medicine of Zhejiang A&F University,Hangzhou 311300,China;Development Center of Animal Husbandry and Agricultural Machinery in Hangzhou Lin'an district,Hangzhou 311300,China;Jiangxi Zhengbang Academy of Agricultural Sciences,Nanchang 330096,China)
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2022年第10期1045-1051,1065,共8页
Chinese Journal of Preventive Veterinary Medicine
基金
国家自然科学基金(31972656)
浙江省农业重大技术协同推广项目(2021XTTGXM02-05)
浙江省三农六方项目(2021SNLF023)
杭州市临安区畜牧业转型发展项目(2021-197-04)
全国大学生创新创业训练项目(202010341038)。
关键词
副猪嗜血杆菌
链球菌
血清型
耐药基因
流行趋势
Haemophilus parasuis
Streptococcus Suis
serotypes
drug resistant genes
epidemic trend
