摘要
目的观察腹部推拿对溃疡性结肠炎大鼠肠道炎症及脊髓背角蛋白激酶B(AKT)蛋白表达的影响,探讨腹部推拿治疗溃疡性结肠炎的作用机制。方法将50只雄性SD大鼠随机分成正常组、模型组、推拿组和美沙拉嗪组,除正常组外,其余组自由饮用5%葡聚糖硫酸钠溶液7 d制备溃疡性结肠炎大鼠模型。推拿组造模第2日开始腹部推拿干预,每日1次,每次10 min;美沙拉嗪组予0.035 g/mL美沙拉嗪药液灌胃,每日1次,连续15 d。观察大鼠一般状况,进行疾病活动指数(DAI)评分,HE染色观察结肠组织病理变化,Western blot检测脊髓背角AKT、p-AKT表达。结果与正常组比较,模型组大鼠倦怠少动,皮毛黯淡干枯,形体瘦削,可见脓血便,第6日起DAI评分明显升高(P<0.01),结肠上皮细胞破损,黏膜充血、水肿,腺体破坏,杯状细胞减少,有大量炎症细胞浸润,脊髓背角p-AKT蛋白表达明显升高(P<0.05);与模型组比较,推拿组和美沙拉嗪组大鼠皮毛光泽恢复,无脓血便,第9日起DAI评分明显降低(P<0.05),结肠组织病理损伤有不同程度改善,充血、水肿明显减轻,腺体、杯状细胞增多,炎性细胞浸润明显减少,脊髓背角p-AKT蛋白表达明显降低(P<0.05)。各组大鼠脊髓背角AKT蛋白表达差异无统计学意义(P>0.05)。结论腹部推拿能有效改善溃疡性结肠炎大鼠结肠炎症状态,其机制可能与抑制脊髓背角p-AKT表达有关。
Objective To observe the effects of abdominal massage on intestinal inflammation and the expression of protein kinase B(AKT)protein in spinal dorsal horn of rats with ulcerative colitis(UC);To explore the mechanism of abdominal massage in the treatment of UC.Methods Totally 50 male SD rats were randomly divided into normal group,model group,massage group and mesalazine group.Except for the normal group,the other groups were given5%sodium glucan sulfate solution for 7 days to prepare the rat model of UC.The massage group began abdominal massage intervention on the second day of modeling,once a day,10 min each time;mesalazine group was given 0.035 g/mL mesalazine solution intragastric administration,once a day,for consecutive 15 days.The general condition of the rats was observed,and DAI score was performed,HE staining was used to observe the pathological changes of colon tissue,and Western blot was used to detect the expression of AKT and p-AKT protein in spinal dorsal horn.Results Compared with the normal group,the rats in model group were listless and less active,the fur was dim and dry,the body were thin,and pus and blood stools were observed,the DAI score increased significantly from the 6th day(P<0.01),the colon epithelial cells were damaged,mucosal hyperemia and edema,glands were destroyed,goblet cells decreased,and inflammatory cells were infiltrated,the expression of p-AKT protein in spinal dorsal horn significantly increased(P<0.05).Compared with the model group,the fur gloss of the rats in massage group and mesalazine group was restored,without pus and blood stools,the DAI score significantly decreased from the 9th day(P<0.05),the pathological injury of colon tissue was improved to varying degrees,congestion and edema were significantly reduced,glands and goblet cells increased,inflammatory cell infiltration significantly reduced,the expression of p-AKT protein in spinal dorsal horn significantly decreased(P<0.05).There was no significant difference in the expression of AKT in spinal dorsal horn of rats among all groups(P>0.05).Conclusion Abdominal massage can effectively improve the inflammatory state of the colon in rats with UC,and the mechanism may be related to the inhibition of p-AKT expression in spinal dorsal horn.
作者
方佳钰
黄红叶
王晓华
陈水金
江煜
陈乐春
张幻真
陈进城
林志刚
FANG Jiayu;HUANG Hongye;WANG Xiaohua;CHEN Shuijin;JIANG Yu;CHEN Lechun;ZHANG Huanzhen;CHEN Jincheng;LIN Zhigang(Fujian University of Traditional Chinese Medicine,Fuzhou 350100,China;Fujian University of Chinese Medicine Subsidiary Rehabilitation Hospital,Fuzhou 350003,China;Fujian Provincial Key Laboratory of Rehabilitation Technology,Fuzhou 350003,China)
出处
《中国中医药信息杂志》
CAS
CSCD
2023年第1期109-113,共5页
Chinese Journal of Information on Traditional Chinese Medicine
基金
国家自然科学基金(82004494)
福建省自然科学基金(2020J01757)
福建省康复技术重点实验室项目(KF2019021)。
