摘要
糖外排转运蛋白(sugars will eventually be exported transporters,SWEET)介导植物光合同化产物蔗糖的跨膜运输.以橄榄(Canarium album(Lour.)Raeusch.)果实为材料,通过气相色谱串联质谱(GC-MS)检测橄榄果实中糖组分及含量变化,利用RT-PCR技术克隆得到CaSWEET7和CaSWEET15基因开放阅读框(ORF)序列.结果表明:CaSWEET7和CaSWEET15基因ORF全长分别为774 bp和951 bp,分别编码长度为257个和316个氨基酸残基,具有2个MtN3_slv结构域;进化树分析表明,CaSWEET7属于CladeⅡ,CaSWEET15属于CladeⅢ.实时荧光定量PCR(qRT-PCR)技术检测其在不同发育时期的果实中相对表达量变化,结果表明,随着果实发育,CaSWEET7和CaSWEET15表达量逐渐递增,且与果实发育蔗糖含量变化呈显著正相关(相关性系数分别为0.931和0.904,P<0.05);利用酵母功能互补证明CaSWEET7和CaSWEET15基因编码的蛋白具有转运蔗糖能力.本研究表明CaSWEET7和CaSWEET15基因可能在橄榄果实蔗糖积累中发挥作用.(图9表2参44)
SWEET(sugars will eventually be exported as transporters) proteins mediate the transmembrane transport of sucrose, a plant photochemical product. In this study, Chinese olive(Canarium album(Lour.)Raeusch.) fruit was used as the material, and the sugar components and content changes in the Chinese olive fruits were detected using gas chromatography-tandem mass spectrometry(GC-MS). The open reading frame(ORF) sequences of CaSWEET7 and CaSWEET15 were cloned using RT-PCR. The results showed that the ORF sequences of CaSWEET7 and CaSWEET15 were 774 bp and 951 bp, respectively, and encoded 257and 316 amino acids, respectively. They had two MtN3_slv domains. Phylogenetic tree analysis showed that CaSWEET7 belonged to clade II, and CaSWEET15 belonged to clade III. Real-time fluorescent quantitative PCR(qRT-PCR) detected its relative expression levels in fruits at different developmental stages and showed that the expression of CaSWEET7 and CaSWEET15 increased gradually with fruit development and was significantly correlated with the change in sucrose content, with correlation coefficients of 0.931 and 0.904,respectively(P < 0.05). The proteins encoded by CaSWEET7 and CaSWEET15 have been shown to have sucrose transport capacity in a yeast functional complementarity assay. This study suggests that CaSWEET7and CaSWEET15 may play a role in sucrose accumulation in Chinese olive fruit.
作者
刘林敏
邱子文
叶清华
谢倩
王威
陈清西
LIU Linmin;QIU Ziwen;YE Qinghua;XIE Qian;WANG Wei;CHEN Qingxi(College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou 350002,China)
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2022年第6期1503-1509,共7页
Chinese Journal of Applied and Environmental Biology
基金
中央引导地方科技发展专项项目(2017L3001)
福建农林大学科技创新专项基金项目(CXZX2018087)
福建农林大学横向科技创新基金项目(102-KHF200005)
福建农林大学科技创新专项基金项目(CXZX2017186)资助。
关键词
橄榄
糖转运蛋白
SWEET基因
表达分析
酵母功能互补试验
Chinese olive(Canarium album)
sugar transporter
SWEET
expression analysis
yeast function complementation assay
