摘要
[目的]探讨姜黄素对膝骨性关节炎(knee osteoarthritis,KOA)疼痛与软骨退变的影响。[方法]30只雄性SD大鼠,随机分为3组,每组10只,假手术组10只动物仅切开关节囊,其余20只动物切断前交叉韧带离断法建立KOA大鼠模型。姜黄素组给予姜黄素(50 mg/kg)腹腔注射,假手术组和模型组给予等量生理盐水注射,1次/d,连续8周。建模术后8周,评价疼痛反应,行组织学观察与评分,ELESIA法检测滑液IL-1β、TNF-α,MCP-1含量,Western blot法检测软骨MMP-1、MMP-13、Aggrecan和CollagenⅡ。[结果]与假手术组相比,模型组的MWT和TWL均显著降低(P<0.05),而OARSI组织学评分显著增加(P<0.05);与模型组相比,姜黄素组MWT、TWL显著升高(P<0.05),OARSI评分显著降低(P<0.05)。ELESIA检测方面,与假手术组相比,模型组IL-1β、TNF-α、MCP-1含量均显著升高(P<0.05);与模型组相比,姜黄素组IL-1β、TNF-α、MCP-1含量均显著降低(P<0.05)。Western blot检测方面,与假手术组相比,模型组软骨组织MMP-1、MMP-13蛋白显著升高,而Aggrecan和CollagenⅡ蛋白显著降低(P<0.05);与模型组相比,姜黄素组MMP-1、MMP-13显著降低,而而Aggrecan和CollagenⅡ显著升高(P<0.05)。[结论]姜黄素可以显著减轻KOA大鼠膝关节的痛觉过敏和软骨退变,其作用机制可能与减少IL-1β、TNF-α,MCP-1的含量及恢复软骨代谢平衡有关。
[Objective]To investigate the effects of curcumin on pain and cartilage degeneration of knee osteoarthritis(KOA)in rat.[Methods]Thirty male Sprague-Dawley rats were randomly divided into 3 groups with 10 rats in each group.Ten animals in the sham group received capsulotomy only,while the remaining 20 rats had anterior cruciate ligament resected to establish the KOA rat model.Ten rats in the curcumin group was given curcumin(50 mg/kg)intraperitoneally,while the other 20 animals in the sham and model groups were given the same amount of normal saline once a day for 8 weeks.Eight weeks after modeling,pain response was evaluated,histological observation and scoring were conducted.In addition,ELESIA assay was used for synovial Il-1β,TNF-αand McP-1,while Western blot assay was conducted for MMP-1,MMP-13,Aggrecan and Collagen II of cartilage.[Results]The model group had significantly lower mechanical withdrawal threshold(MWT)and thermal withdrawal latency(TWL)(P<0.05),while significantly higher Osteoarthritis Research Society International(OARSI)histological score than the sham group(P<0.05).However,the curcumin group got significantly reversed variation in terms of MWT,TWL and OARSI scores compared with those in the model group(P<0.05).As results of ELESIA assay,the model group had significantly higher synovial Il-1β,TNF-αand MCP-1 than the sham group(P<0.05),whereas which were significantly reversed in the curcumin group(P<0.05).In term of Western blot assay,the model group had significant increases in MMP-1 and MMP-13,whereas significant decreases in aggrecan and collagen II compared with the sham group(P<0.05).Compared with model group,the curcumin group got significantly decreased MMP-1 and MMP-13(P<0.05),while significantly increased aggrecan and collagen II(P<0.05).[Conclusion]The curcumin does considerably reduce hyperalgesia and cartilage degeneration in the KOA rats,which may be related to reducing IL-1β,TNF-αand McP-1 and restoring the balance of cartilage metabolism.
作者
孙秀钦
秦晓彬
李含
张超远
叶向阳
SUN Xiu-qin;QIN Xiao-bin;LI Han;ZHANG Chao-yuan;YE Xiang-yang(Department of Orthopaedics,Nanyang Central Hospital,Nanyang 473000,China)
出处
《中国矫形外科杂志》
CAS
CSCD
北大核心
2022年第23期2163-2167,共5页
Orthopedic Journal of China
基金
河南省重点研发与推广专项(科技攻关)项目(编号:182102310466)。
关键词
膝骨性关节炎
疼痛
软骨退变
姜黄素
knee osteoarthritis
pain
cartilage degeneration
curcumin
