摘要
目的:基于网络药理学研究香附治疗女性青春期后痤疮的作用机制。方法:通过TCMSP数据库获取香附的活性成分与相应靶点,采用GeneCard数据库获得与痤疮相关的疾病靶点,使用STRING数据库和Cytoscape 3.7.1软件构建蛋白质-蛋白质相互作用(PPI)网络,应用Bioconductor数据库进行基因本体(GO)功能、京都基因与基因组百科全书(KEGG)通路富集分析。结果:香附的有效活性成分共18种,痤疮相关靶点570个,潜在靶点21个,药物-疾病靶点21个。PPI网络显示,白细胞介素-6、血管内皮生长因子A、白三醇过氧化物酶体增殖物激活受体γ、表皮生长因子受体、白三烯醇雌激素受体的连接程度最高。GO功能富集分析结果显示,交集基因主要参与了整合素结合、核受体活性、配体激活转录因子活性、ATP酶结合等。KEGG通路主要涉及脂质与动脉粥样硬化、流体剪切应力与动脉粥样硬化、肿瘤坏死因子信号通路、磷脂酰肌醇-3激酶-蛋白激酶B(PI3K-Akt)信号通路等。结论:香附可通过调节多种生物学功能和各项通路治疗女性青春期后痤疮,为临床运用香附治疗女性青春期后痤疮提供了理论支持。
Objective:To investigate the mechanism of action of Rhizoma Cyperi in the treatment of women with post-adolescent acne based on network pharmacology.Methods:TCMSP database was used to obtain the active components of Rhizoma Cyperi and corresponding targets,and GeneCard database was used to obtain the disease targets associated with acne;STRING database and Cytoscape 3.7.1 software were used to construct a protein-protein interaction(PPI)network,and Bioconductor database was used to perform gene ontology(GO)functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis.Results:There were 18 active components of Rhizoma Cyperi,570 targets associated with acne,21 potential targets,and 21 drug-disease targets.The PPI network showed that interleukin-6,vascular endothelial growth factor A,Baisanchun peroxisome proliferator-activated receptorγ,epidermal growth factor receptor,and Baisanxichun estrogen receptor showed the highest degree of connection.GO functional enrichment analysis showed that the intersecting genes were mainly involved in integrin binding,nuclear receptor activity,ligand-activated transcription factor activity,and ATPase binding.KEGG pathways were mainly involved in lipids and athero-sclerosis,fluid shear stress and atherosclerosis,the tumor necrosis factor signaling pathway,and the PI3K-Akt signaling pathway.Conclusion:Rhizoma Cyperi exerts a therapeutic effect on post-adolescent acne in women by regulating various biological functions and pathways,which provides a theoretical basis for the clinical application of Rhizoma Cyperi in the treatment of post-adolescent acne in women.
作者
张园园
隋秀林
魏淑相
ZHANG Yuanyuan;SUI Xiulin;WEI Shuxiang(Weifang Hospital of Traditional Chinese Medicine,Weifang 261041,Shandong,China;Shandong University of Traditional Chinese Medicine,Jinan 250355,Shandong,China)
出处
《湖南中医杂志》
2022年第11期178-183,共6页
Hunan Journal of Traditional Chinese Medicine
基金
山东省潍坊市卫生健康委员会中医药科研计划项目(第2020-2-005号)。
关键词
网络药理学
香附
青春期后痤疮
GO功能富集分析
KEGG通路富集分析
network pharmacology
Rhizoma Cyperi
post-adolescent acne
gene ontology functional enrichment analysis
Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis
