摘要
目的 研究华南忍冬Lonicera confusa干燥花蕾(山银花)的化学成分。方法 综合应用HP-20、硅胶、ODS、Sephadex LH-20和半制备液相等多种色谱学方法进行分离纯化,并根据化合物的理化性质和核磁共振波谱数据进行结构鉴定。采用LPS诱导小鼠巨噬细胞RAW264.7模型,以抑制炎性细胞因子一氧化氮(NO)的分泌为评价指标对分离得到的化合物进行体外抗炎活性评价。结果 从华南忍冬干燥花蕾70%乙醇提取物中分离鉴定了5个化合物,分别为8-[α-L-arabinopyranosyl-(1′′→6′)-β-D-glucopyranosyl]-2,6-dimethyloct-1,2′′-lactone(1)、天师酸(2)、黄麻脂肪酸F(3)、天师酸甲酯(4)和黄麻脂肪酸F甲酯(5)。结论 化合物1为新的单萜苷类化合物,命名为忍冬单萜A,化合物2~5为首次从忍冬属植物中分离得到。化合物对LPS诱导的RAW264.7细胞中NO的分泌均无显著的抑制作用。
Objective To study the constituents from the dried flower buds of Lonicera confusa.Methods The compounds were isolated and purified by column chromatography of HP-20 macroporous resin,silica gel,ODS,Sephadex LH-20,and semi-preparative RP-HPLC.Their structures were elucidated by physicochemical properties and spectral analyses.Taking NO as the evaluating indicator,the model of LPS-induced RAW264.7 cells was used to evaluate the in vitro anti-inflammatory activity of isolates.Results Five compounds were isolated and identified from the 70% ethanol extract of L.confusa,including 8-[α-L-arabinopyranosyl-(1′′→6′)-β-D-glucopyranosyl]-2,6-dimethyloct-1,2′′-lactone(1),tianshic acid(2),corchorifatty acid F(3)、tianshic acid methyl ester(4) and corchorifatty acid F methyl ester(5),respectively.Conclusion Compound 1 is a new monoterpene glycoside,named comfusanoside A.Compounds 2—5 are identified from Lonicera for the first time.All the isolates had no significant inhibitory effect on NO secretion in RAW264.7 cells stimulated by LPS.
作者
王团委
王振中
王秋红
耿剑亮
WANG Tuan-wei;WANG Zhen-zhong;WANG Qiu-hong;GENG Jian-liang(School of Traditional Chinese Medicine Guangdong Pharmaceutical University,Guangzhou 510006,China;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process,Kanion Pharmaceutical Co.,Ltd.,Lianyungang222001,China;Key Laboratory for the New Technique Research of TCM Extraction and Purification,Kanion Pharmaceutical Co.,Ltd.,Lianyungang 222001,China;Nanjing University of Chinese Medicine,Nanjing 210023,China)
出处
《中草药》
CAS
CSCD
北大核心
2022年第23期7317-7323,共7页
Chinese Traditional and Herbal Drugs
基金
国家中医药管理局中央财政转移支付地方项目:基于重点研究室研究领域的中医药多学科研究能力提升项目-中药提取精制新技术。
