摘要
外来入侵物种曲纹紫灰蝶Chilades pandava是危害苏铁属的重要害虫。从植物中寻找驱避活性成分为曲纹紫灰蝶的绿色防控提供基础支撑。试验采用水蒸气蒸馏法提取番石榴Psidium guajava叶精油,对其化学组成进行气相色谱-质谱联用(GC-MS)分析,采用Y型嗅觉仪和气相色谱-触角电位联用仪(GC-EAD)测定精油及其主要成分对曲纹紫灰蝶雌成虫的驱避作用和触角电位活性。研究结果表明,番石榴叶精油含有大量的倍半萜及其氧化物,主要是(E)-β-石竹烯(32.47%)、α-古巴烯(10.44%)、菖莆烯(8.19%)、蓝桉醇(7.19%)和香橙烯(5.01%)等。曲纹紫灰蝶对番石榴叶精油中的8种成分有触角电位反应,其中对(E)-β-石竹烯的反应最明显。番石榴叶精油和(E)-β-石竹烯均能驱避曲纹紫灰蝶,驱避率与浓度呈正相关,在0.5μL/mL时,驱避率分别为73.08%和68.00%。
The invasive alien species Chilades pandava is a major insect pest of Cycas spp. plants.Repellents from non-host plants provide a basis for the green control of C. pandava. In this study, the essential oil of Psidium guajava leaves(PGEOs) was extracted with the steam distillation method, and its chemical composition was analyzed using gas chromatography-mass spectrometry(GC-MS). The repellent effect and the electroantennographic activity of PGEOs as well as its main components on the females of C. pandava were determined with the Y-olfactometer and the gas chromatography-electroantennographic detection(GC-EAD)respectively. The results from the GC-MS analysis showed that PGEOs mainly contained sesquiterpenes and oxygenated sesquiterpenes, including(E)-β-caryophyllene(32.47%), α-copaene(10.44%), calamenene(8.19%),globulol(7.19%) and aromandendrene(5.01%). GC-EAD recordings confirmed the electrophysiological activity of eight volatile compounds in PGEOs, of which(E)-β-caryophyllene was the most active compound and had a dose-dependent effect. PGEOs and(E)-β-caryophyllene can both significantly repel C. pandava females with dose-dependent, and the repellent rates were 73.08% and 68.00%, respectively, at the concentration of 0.5 μL/mL.
作者
黄华
凌斯全
邱华龙
钟家美
徐金柱
HUANG Hua;LING Siquan;QIU Hualong;ZHONG Jiamei;XU Jinzhu(Guangdong Provincial Key Laboratory of Silviculture,Protection and Utilization/Guangdong Academy of Forestry,Guangzhou,Guangdong 510520;ChinaForest Pest Control and Quarantine station,Fengkai County Forestry Bureau,Zhaoqing,Guangdong 526500,China)
出处
《林业与环境科学》
2022年第6期7-14,共8页
Forestry and Environmental Science
基金
广东省林业科技创新项目(2019KJCX025)
广东省基础与应用基础研究基金(2019A1515110596)。
关键词
曲纹紫灰蝶
植物精油
化学成分
驱避活性
触角电位
Chilades pandava
essential oils
chemical constituents
repellent activity
electroantennogram
