摘要
目的 基于RP-HPLC-DAD指纹图谱结合化学计量学方法,评价不同品种木豆Cajanus cajan叶质量特征的共有性和差异性,为木豆叶的质量评价提供科学依据。方法 通过RP-HPLC-DAD建立13个不同品种木豆叶的RP-HPLC-DAD指纹图谱,进行相似度分析(similarity analysis,SA),结合聚类分析(hierarchical cluster analysis,HCA)、主成分分析(principal component analysis,PCA)和最小二乘判别分析(partial least squares discriminant analysis,PLS-DA)对木豆叶质量进行综合评价,并对样品中6种主要成分进行含量测定。结果 木豆叶RP-HPLC-DAD指纹图谱共标定13个共有峰,13个品种木豆叶的相似度均在0.90以上,通过HCA可将这13个品种的木豆叶分为5类。PCA与HCA结果基本一致,经过PCA分析发现S8的综合得分最高,质量最好,其次是S9和S12。PLS-DA与PCA结果基本一致,不同品种木豆叶的化学成分差异主要由7、10、6、9、11号5个色谱峰引起的。S8、S9和S12的含量处于前3,与PCA分析的综合得分一致。结论 指纹图谱结合HCA、PCA和PLS-DA可以全面的评价木豆叶质量,为木豆叶的质量控制提供全面的参考。
Objective To evaluate the commonality and difference of Cajanus cajan leaves based on RP-HPLC-DAD fingerprints combined with chemometric methods,providing a scientific basis for the quality evaluation of C.cajan leaves.Methods The RP-HPLCDAD fingerprints of 13 different species of C.cajan leaves were established by RP-HPLC-DAD.The quality of C.cajan leaves.was evaluated by similarity analysis(SA),hierarchical cluster analysis(HCA),principal component analysis(PCA) and partial least square discriminant analysis(PLS-DA) and six main components in the sample were determined by RP-HPLC-DAD.Results The HPLC fingerprint of C.cajan leaves was calibrated with 13 common peaks,and the similarity of them were all above 0.90.These 13 species of C.cajan leaves were divided into five categories by HCA.The results of PCA and HCA were basically consistent.According to PCA,S8had the highest comprehensive score and the best quality,followed by S9 and S12.The results of PLS-DA and PCA were basically consistent.The differences in composition of C.cajan leaves were mainly caused by chromatographic peaks 7,10,6,9,and 11.The content of S8,S9 and S12 were among the top 3,which was consistent with the comprehensive score of PCA.Conclusion Fingerprint combined with HCA,PCA and PLS-DA can comprehensively evaluate the quality of C.cajan leaves and provide a comprehensive reference for quality control of C.cajan leaves.
作者
符丽娜
王立涛
王建栋
王燕秋
蔡子辉
闫鑫宇
赵棚权
张谡
付玉杰
FU Li-na;WANG Li-tao;WANG Jian-dong;WANG Yan-qiu;CAI Zi-hui;YAN Xin-yu;ZHAO Peng-quan;ZHANG Su;FU Yu-jie(College of Forestry,Beijing Forestry University,Beijing 100083,China)
出处
《中草药》
CAS
CSCD
北大核心
2022年第24期7880-7886,共7页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金重点项目(31930076)
通络生骨胶囊品质提升研究(2020HXFWLXY001)。
关键词
木豆叶
指纹图谱
化学计量学
聚类分析
主成分分析
最小二乘判别分析
荭草苷
异牡荆苷
牡荆苷
木犀草苷
质量评价
leaves of Cajanus cajan(L.)Millsp
fingerprint
chemometrics
cluster analysis
principal component analysis
partial least square discriminant analysis
orientin
isovitexin
vitexin
luteoloside
quality evaluation
