摘要
为了比较牟氏角毛藻(Chaetoceros muelleri)、蛋白核小球藻(Chlorella pyrenoidosa)、三角褐指藻(Phaeodactylum tricornutum)、新月柱鞘藻(Nitzschia closterium)四株微藻在悬浮和固定培养方式下藻细胞生长状况和氮磷去除效果,试验采取模拟含氮磷人工污水,向培养液中添加NH4Cl,在相同培养条件下,分别培养4株微藻。实验结果表明:4株微藻在悬浮和固定化培养均能生长,氮磷去除效果良好。4种微藻悬浮培养的生长速率要优于固定化培养。两种培养方式生长速率均为:蛋白核小球藻>新月柱鞘藻>三角褐指藻>牟氏角毛藻;4种藻在悬浮培养下,NH_(4)^(+)-N和PO_(4)^(3-)-P去除率为:新月柱鞘藻>三角褐指藻>牟氏角毛藻>蛋白核小球藻;4种藻在固定化培养下,NH_(4)^(+)-N和PO_(4)^(3-)-P去除率为:新月柱鞘藻>三角褐指藻>蛋白核小球藻>牟氏角毛藻。除牟氏角毛藻外,蛋白核小球藻、新月柱鞘藻和三角褐指藻的NH_(4)^(+)-N去除率是固定化培养优于悬浮培养,PO_(4)^(3-)-P去除率均为悬浮培养均优于固定化培养。综合上所述,4种微藻固定化后均对其生长速率和氮磷去除效果产生不同程度的影响,其中新月柱鞘藻固定化培养生长和氮磷去除效果最佳,最适合作为固定化培养方式氮磷高效去除的藻株。
Microalgae can survive and proliferate in waste water containing high-concentration nitrogen and phosphate from municipal or agricultural sewage,as well as absorb and assimilate for NH_(4)^(+)-N and PO_(4)^(3-)-P.The cultivation of microalgae in immobilization has more significant strength than in suspension.The experiment set the same culture environment and imitated artificial sewage of nitrogen and phosphate using ammonium chloride and monopotassium phosphate,thus the growth rate and the removal rate of nitrogen and phosphorus of suspended and immobilized marine microalgae (Chaetoceros muelleri,Chlorella pyrenoidosa,Phaeodactylum tricornutum and Nitzschia closterium) were compared.The results showed that these microalgae could grow in suspended and immobilized cultivation,and had superior removal efficiencies of NH_(4)^(+)-N and PO_(4)^(3-)-P.The growth rate of these marine microalgae in suspended was better than in immobilized.The growth rates of the two culture methods:Chlorella pyrenoidosa>Nitzschia closterium>Phaeodactylum tricornutum>Chaetoceros muelleri.The removal rates of NH_(4)^(+)-N and PO_(4)^(3-)-P in suspended were as follows:Nitzschia closterium>Phaeodactylum tricornutum>Chaetoceros muelleri>Chlorella pyrenoidosa;the removal rates of NH_(4)^(+)-N and PO_(4)^(3-)-P in immobilized were as follows:Nitzschia closterium>Phaeodactylum tricornutum>Chlorella pyrenoidosa>Chaetoceros muelleri.Except Chaetoceros muelleri,the removal rates of NH_(4)^(+)-N in immobilized including Nitzschia Closterium,Phaeodactylum tricornutum and Chlorella pyrenoidosa were better than those of suspension cultivation.The removal of PO_(4)^(3-)-P in immobilized had higher efficiencies than in suspended.In summary,the growth rates and removal rates of NH_(4)^(+)-N and PO_(4)^(3-)-P were inferior to in the immobilized culture.Nitzschia Closterium exhibited enormous advantages in growing and removal rates of NH_(4)^(+)-N and PO_(4)^(3-)-P under immobilization.
作者
张聪颖
薛瑞萍
彭瑞冰
韩庆喜
蒋霞敏
ZHANG Congying;XUE Ruiping;PENG Ruibing;HAN Qingxi;JIANG Xiamin(School of Marine Sciences,Ningbo University,Ningbo 315211,China)
出处
《生态科学》
CSCD
2023年第1期76-82,共7页
Ecological Science
基金
浙江省省级科技计划项目(2019C2055)。
关键词
微藻
悬浮
固定化
氮磷
microalgae
suspension
immobilization
nitrogen and phosphorus
