摘要
目的观察蒙药那仁满都拉对去卵巢骨质疏松模型大鼠骨微结构与骨形成的影响,初步探讨其防治骨质疏松的作用及机制。方法将30只雌性大鼠随机分为假手术组、模型组、蒙药那仁满都拉低、中、高剂量组,每组6只,利用去卵巢大鼠构建骨质疏松模型大鼠,模型制备成功后用蒙药灌胃,假手术组用相同体积的生理盐水灌胃。连续12周后,通过对股骨组织进行Micro-CT、骨骼力学检测、HE染色,分别观察大鼠骨密度、骨骼力度、骨组织病理形态,检测Wnt、β-catenin蛋白表达情况;从1日龄大鼠颅骨中提取成骨细胞用低、中、高浓度的含药血清干预后检测ALP染色、矿化染色,观察其ALP分泌、矿化结节大小,检测Wnt、β-catenin蛋白表达情况。结果在模型大鼠股骨组织中Micro-CT显示,蒙药组均能增加骨小梁密度;骨骼力学分析显示,蒙药组均能提高骨骼力学的载荷与刚度,其中中、高剂量组具有显著统计学意义(P<0.01,P<0.05)。HE染色结果显示蒙药组均可明显改善骨小梁病理变化;Western blot结果提示蒙药组中Wnt、β-catenin蛋白表达明显升高(P<0.0001);蒙药含药血清组均能促进成骨细胞碱性磷酸酶分泌,矿化结节形成和Wnt、β-catenin蛋白表达(P<0.0001)。结论蒙药那仁满都拉可以改善去卵巢骨质疏松模型大鼠的骨微结构,其发挥抗骨质疏松作用可能与调节Wnt/β-catenin信号通路有关。
Objective The effect of Mongolian medicine Narenmandula on bone microstructure and bone formation in ovariectomized osteoporosis rat model was observed,and its anti-osteoporosis effect and mechanism were preliminarily discussed.Methods Thirty female rats were randomly divided into sham surgery group,model group,and Mongolian medicine Narenmandu low-,medium-,and high-dose group,with 6 rats per group.Osteoporosis model rats were established with ovariectomy.After successful model preparation,rats in Mongolian medicine group received Mongolian medicine with gastric irrigation.Rats in sham surgery group received the same volume of normal saline.After 12 consecutive weeks,micro-CT,bone mechanics,and HE staining were performed on femurs.The expressions of Wnt andβ-catenin proteins was detected in rats,respectively.ALP staining and mineralization staining were detected after intervention with low-,medium-,and high-concentrations of drug-containing serum from 1-day-old rat skulls.The ALP secretion and mineralized nodule size were observed.The expression of Wnt andβ-catenin proteins was detected.Results Micro-CT result showed that the density of bone trabecular in the Mongolian drug group increased in the model rat femurs.The bone mechanical analysis showed that the load and stiffness of bone mechanics improved in the Mongolian medicine group,and they were significant the high-and middle-dose group(P<0.01,P<0.05).The result of HE staining showed that the pathological changes of trabecular bone were significantly improved in the Mongolian drug groups.Western blotting result suggested that the expressions of Wnt andβ-catenin proteins in the Mongolian drug groups increased significantly(P<0.0001).The Mongolian drug-containing serum promoted alkaline phosphatase secretion,mineralized nodule formation,and Wnt andβ-catenin protein expressions in the Mongolian drug groups(P<0.0001).Conclusion Mongolian medicine Narenmandula improves the bone microstructure of rats in the ovariectomized osteoporosis model.Its anti-osteoporosis effect may be related to the regulation of the Wnt/β-catenin signaling pathway.
作者
余自层
郭杨
马勇
潘娅岚
韩秋革
肖吉日木图
刘孟敏
YU Ziceng;GUO Yang;MA Yong;PAN Yalan;HAN Qiuge;XIAO Jirimutu;LIU Mengmin(School of Chinese Medicine,School of Integrated Chinese and Western Medicine,Nanjing University of Chinese Medicine,Nanjing 210023;Laboratory of New Techniques of Restoration&Reconstruction of Orthopedics and Traumatology,Nanjing University of Chinese Medicine,Nanjing 210023;Nursing Institute of Integrated Chinese and Western Medicine,Nanjing University of Chinese Medicine,Nanjing 210029;Inner Mongolia Medical University,Hohhot 010110,China)
出处
《中国骨质疏松杂志》
CAS
CSCD
北大核心
2023年第1期58-63,69,共7页
Chinese Journal of Osteoporosis
基金
内蒙古自治区自然科学基金面上项目(2020MS08134)
江苏省高校自然科学创新计划项目(KYCX22_1881,KYCX22_1922)
江苏高校中西医临床医学品牌专业建设工程资助项目(2020PPZXL261)。
