摘要
目的:分析长链非编码RNA(LncRNA)癌易感性候选基因2(CASC2)、miR-155-5p与上皮性卵巢癌病人化疗敏感性及预后的相关性关系。方法:采用实时荧光定量PCR(qRT-PCR)法检测87例上皮性卵巢癌病人(上皮性卵巢癌组)及72例卵巢良性肿瘤病人(对照组)LncRNA CASC2、miR-155-5p表达水平,比较2组LncRNA CASC2、miR-155-5p表达水平,分析上皮性卵巢癌组LncRNA CASC2、miR-155-5p表达水平与临床病理特征的相关性关系。根据上皮性卵巢癌组病人术后化疗是否出现继发耐药分为化疗耐药组(n=32例)和化疗敏感组(n=55例),分析LncRNA CASC2、miR-155-5p表达水平与化疗敏感性的关系。同时采用Pearson法分析上皮性卵巢癌组织中LncRNA CASC2与miR-155-5p的相关性关系,采用Kaplan-Meier法分析两者与上皮性卵巢癌病人5年生存情况的关系。结果:上皮性卵巢癌组LncRNA CASC2表达水平低于对照组,而miR-155-5p表达水平高于对照组,差异均有统计学意义(P<0.01);上皮性卵巢癌组LncRNA CASC2、miR-155-5p表达水平与腹腔转移、FIGO分期、分化程度及淋巴结转移有关(P<0.05~P<0.01),而与年龄、肿瘤大小、腹水及组织学类型无关(P>0.05);化疗敏感组miR-155-5p表达水平低于化疗耐药组,而LncRNA CASC2表达水平高于化疗耐药组,差异均有统计学意义(P<0.01);上皮性卵巢癌组LncRNA CASC2与miR-155-5p呈负相关关系(r=-0.637,P<0.05);miR-155-5p高表达者5年内总生存率及中位生存时间均低于miR-155-5p低表达者(P<0.05),而LncRNA CASC2高表达者5年内总生存率及中位生存时间均高于LncRNA CASC2低表达者(P<0.05)。结论:上皮性卵巢癌病人癌组织LncRNA CASC2、miR-155-5p表达水平与化疗敏感性及预后存在密切相关性,可作为有效的预测指标。
Objective:To analyze the correlation between long chain non-coding RNA(LncRNA)cancer susceptibility candidate 2(CASC2),miR-155-5p and chemotherapy sensitivity and prognosis of patients with epithelial ovarian cancer.Methods:The expression levels of LncRNA CASC2 and miR-155-5p in 87 patients with epithelial ovarian cancer(epithelial ovarian cancer group)and 72 patients with benign ovarian cancer(control group)were detected by real-time quantitative PCR(QRT-PCR).The expression levels of LncRNA CASC2 and miR-155-5p between two groups were compared.The correlations between LncRNA CASC2,miR-155-5p expression levels and clinicopathological features were analyzed in the epithelial ovarian cancer group.The epithelial ovarian cancer group was subdivided into the chemotherapy-resistant group(n=32)and chemotherapy-sensitive group(n=55)according to the occurrence of secondary drug resistance after chemotherapy,and the relationship between the expression levels of LncRNA CASC2 and miR-155-5p and chemotherapy sensitivity were analyzed.Meanwhile,the Pearson method was used to analyze the correlation between LncRNA CASC2 and miR-155-5p in epithelial ovarian cancer tissues,and the Kaplan-Meier method was used to analyze the relationship between the LncRNA CASC2,miR-155-5p and 5-year survival of patients with epithelial ovarian cancer.Results:The expression level of LncRNA CASC2 in epithelial ovarian cancer group was lower than that in control group,while the expression level of miR-155-5p in epithelial ovarian cancer group was higher than that in control group(P<0.01).The expression levels of LncRNA CASC2 and miR-155-5p in epithelial ovarian cancer group were correlated with the abdominal metastasis,FIGO stage,differentiation degree and lymph node metastasis(P<0.05 to P<0.01),but not correlated with the age,tumor size,ascites and histological type(P>0.05).The expression level of miR-155-5p in chemotherapy-sensitive group was lower than that in chemotherapy-resistant group,while the expression level of LncRNA CASC2 in chemotherapy-sensitive group was higher than that in chemotherapy-resistant group(P<0.01).The LncRNA CASC2 was negatively correlated with miR-155-5p in epithelial ovarian cancer group(r=-0.637,P<0.05).The 5-year overall survival rate and median survival time in patients with high expression of miR-155-5p were lower than those in patients with low expression of miR-155-5p(P<0.05),while the 5-year overall survival rate and median survival time in patients with high expression of LncRNA CASC2 were higher than those in patients with low expression of LncRNA CASC2(P<0.05).Conclusions:The expression levels of LncRNA CASC2 and miR-155-5p in the cancer tissues of epithelial ovarian cancer patients are closely correlated with the chemotherapy sensitivity and prognosis,and can be used as the effective predictors.
作者
伍玲
杨勇波
WU Ling;YANG Yong-bo(Department of Obstetrics and Gynecology,Kaizhou Hospital of Traditional Chinese Medicine,Chongqing 405400,China;Department of Oncology,Kaizhou Hospital of Traditional Chinese Medicine,Chongqing 405400,China)
出处
《蚌埠医学院学报》
CAS
2023年第2期217-221,共5页
Journal of Bengbu Medical College
