摘要
目的通过对非梗阻性无精子症(NOA)和健康捐精者的血清代谢物进行比较分析,确定与生精功能障碍相关的潜在生物标志物,为NOA的病因诊断和治疗提供依据。方法采用高效液相色谱-串联质谱法检测NOA患者(NOA组,5例)和健康捐精者(对照组,5例)的血清标本。通过血清代谢组学技术分析得到NOA组和对照组的差异代谢物,从中识别出潜在的NOA生物标志物,并分析其可能的代谢途径。结果共筛选并鉴定出21种代谢物可作为NOA的潜在生物标志物,这些代谢物主要与精子发生中的能量产生、氧化应激和细胞凋亡密切相关。此外,NOA患者花生四烯酸代谢、赖氨酸降解、色氨酸代谢、类固醇激素生物合成、萜类骨架合成、叶酸合成等多种代谢途径均受到干扰;FoxO信号通路、cAMP信号通路、mTOR信号通路、PI3K/Akt信号通路等都受到影响。结论NOA的病因可能与血清代谢紊乱有关。血清代谢组学可以识别NOA独特的代谢模式,为男性不育的发病机制提供新的见解。
Objective By comparing and analyzing the serum metabolites of non-obstructive azoospermia(NOA)and healthy sperm donors,identify potential biomarkers related to spermatogenic dysfunction,to provide basis for the etiological diagnosis and treatment of NOA.Methods The serum samples of NOA patients(NOA group,5 cases)and healthy sperm donors(control group,5 cases)were detected by High Performance Liquid Chromatography-Tandem Mass Spectrometry.Serum metabolomics techniques were used to analyze the different metabolites between the NOA group and the control group,from which potential biomarkers of NOA were identified,and possible metabolic pathways were analyzed.Results A total of 21 metabolites were identified as potential biomarkers of NOA.These metabolites related closely to energy production,oxidative stress and apoptosis in spermatogenesis.In addition,arachidonic acid metabolism,lysine degradation,tryptophan metabolism,steroid hormone biosynthesis,terpenoid skeleton synthesis,folic acid synthesis and other metabolic pathways were interfered in NOA patients.FoxO signaling pathway,cAMP signaling pathway,mTOR signaling pathway and PI3K/Akt signaling pathway were all affected.Conclusion The cause of NOA might relates to the disorder of serum metabolism.Serum metabolomics could identify the unique metabolic patterns of NOA and provide new insights into the pathogenesis of male infertility.
作者
彭红波
刘晃
宋小燕
吴瑞珊
孟艳辉
赵文忠
PENG Hongbo;LIU Huang;SONG Xiaoyan;WU Ruishan;MENG Yanhui;ZHAO Wenzhong(Key Laboratory of Male Reproduction and Genetics of National Health Commission,Guangdong Provincial Reproductive Science Institute(Guangdong Provincial Reproductive Hospital),Guangzhou,Guangdong 510600,China)
出处
《检验医学与临床》
CAS
2023年第5期625-629,共5页
Laboratory Medicine and Clinic
基金
广东省医学科研基金项目(B2021405)。
