摘要
【目的】研究线粒体外膜转运酶亚基Tom7基因在尖孢镰刀菌粘团专化型中的功能。【方法】利用同源重组原理,采用PEG介导原生质体的转化方法获得基因敲除突变体,并分析其表型和致病力。【结果】敲除Tom7基因导致尖孢镰刀菌的生长速率减慢,菌丝出现波浪状弯曲生长,产孢量增加,对氧胁迫和渗透胁迫敏感,但对其毒力无影响。【结论】由此推测线粒体外膜转运酶亚基Tom7基因缺失后,影响线粒体蛋白转运功能,进一步对尖孢镰刀菌的生长发育和产孢造成影响。
【Objective】The current study aimed to study the functions of the mitochondrial outer membrane protein transporter subunit Tom7 in Fusarium oxysporum f.sp.conglutinans.【Method】Knockout mutants were obtained using methods of homologous recombination and protoplast-mediated transformation.Then the phenotypes and pathogenicity were analyzed between wild type and mutants.【Result】The deletion mutants showed slower growth rate,wavy curved mycelia,increased sporulation,and more sensitive to oxygen stress and osmotic stress,but had no effect on virulence.【Conclusion】It was speculated that Tom7 played roles in mitochondrial protein transport.Knockout of Tom7 further affected the growth,development and sporulation of Fusarium oxysporum f.sp.conglutinans.
作者
王鑫磊
王碧香
李二峰
WANG Xinlei;WANG Bixiang;LI Erfeng(College of Horticulture and Landscape Architecture,Tianjin Agricultural University,Tianjin 300392,China)
出处
《四川农业大学学报》
CSCD
北大核心
2023年第1期54-60,共7页
Journal of Sichuan Agricultural University
基金
国家自然科学基金项目(31801682)
大学生创新创业训练计划项目(202010061067)。
关键词
尖孢镰刀菌
线粒体外膜转运酶亚基Tom7
基因敲除
表型分析
致病性
Fusarium oxysporum
mitochondrial outer membrane transporter subunit Tom7
gene knockout
phenotypic analysis
pathogenicity
