摘要
目的探讨生、酒五味子对失眠小鼠神经-免疫-内分泌网络的影响及作用机制。方法50只小鼠随机分为空白组、模型组、地西泮组、生五味子组、酒五味子组,每组10只。除空白组外,其余组小鼠腹腔注射甲状腺素溶液建立失眠小鼠模型,每天造模结束后分别灌胃相应剂量的地西泮和生、酒五味子。空白组和模型组给予等体积的生理盐水。观察并记录小鼠的一般状态;记录小鼠总活动距离及直立次数;记录小鼠的脑电和肌电信号,分析睡眠觉醒期(wake)、非快速眼动睡眠期(NREM)和快速眼动睡眠期(REM)所占时间比;检测小鼠脑视交叉上核(SCN)神经递质[γ-氨基丁酸(GABA)、5-羟色胺(5-HT)、多巴胺(DA)、去甲肾上腺素(NE)、皮质醇(CORT)]含量,检测肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)的荧光强度;检测时钟基因Bmal1、生物钟基因Clock、周期基因Per2 mRNA的表达。结果与空白组比较,模型组小鼠精神状态相对萎靡,饮食量和饮水量增加,体质量降低,毛发粗糙无光泽,昼夜节律出现不规律现象;总活动距离、直立次数显著降低;wake所占时间比显著上升,REM、NREM所占时间比显著下降;脑SCN组织中5-HT含量显著降低,NE、DA、CORT含量均显著升高;IL-1β、TNF-α的荧光强度显著升高;Bmal1、Clock mRNA相对表达水平显著升高,Per2 mRNA相对表达水平显著下降(P<0.05或P<0.01)。与模型组比较,生五味子组、酒五味子组小鼠一般状态明显好转,以上指标水平大部分显著逆转(P<0.05或P<0.01)。结论生、酒五味子对失眠小鼠有一定的治疗效果,其机制可能与调控失眠小鼠神经-内分泌-免疫网络相关生物学指标有关。
OBJECTIVE To investigate the effect of raw and wine-processed Schisandra chinensis on neuro-immuneendocrine network in insomnia mice and its mechanism.METHODS Fifty mice were randomly divided into blank group,model group,diazepam group,raw S.chinensis group and wine-processed S.chinensis group,with 10 mice in each group.Except for blank group,the mice in the other groups were intraperitoneally injected with thyroxine solution to establish mice model of insomnia;at the end of each day’s modeling,the corresponding doses of diazepam,raw and wine-processed S.chinensis were given by gavage.The blank group and model group were given constant volume of normal saline.The general state of the mice was observed and recorded,and the total activity distance and upright times of the mice were detected;the EEG and EMG signals of mice were recorded,and the time ratio of sleep wake time(wake),non-rapid eye movement(NREM)and rapid eye movement(REM)was analyzed;the contents of neurotransmitters[γ-aminobutyric acid(GABA),5-hydroxytryptamine(5-HT),dopamine(DA),norepinephrine(NE),cortisol(CORT)]in brain suprachiasmatic nucleus(SCN)were detected;and the expressions of tumor necrosis factorα(TNF-α)and interleukin 1β(IL-1β)were detected;the mRNA expressions of clock gene Bmal1,circadian clock gene Clock and cycle gene Per2 were all detected.RESULTS Compared with the blank group,the mental state of the model group mice was relatively depressed,the amount of food and water increased,the body mass decreased,the hair was rough and shiny,and the circadian rhythm was irregular;the total activity distance and upright times decreased significantly;the time ratio of wake increased significantly,while the time ratios of REM and NREM decreased significantly;the content of 5-HT in brain SCN decreased significantly,while the content of NE,DA and CORT increased significantly;the fluorescence intensity of IL-1βand TNF-αwas significantly increased;the relative expression level of Bmal1 and Clock mRNA was significantly increased,while the relative expression level of Per2 mRNA was significantly decreased(P<0.05 or P<0.01).Compared with the model group,the general state of mice in diazepam group,raw S.chinensis group and wine-processed S.chinensis group was improved obviously,and most of the above index levels were significantly reversed(P<0.05 or P<0.01).CONCLUSIONS Raw and wine-processed S.chinensis have a certain therapeutic effect on insomnia mice,the mechanism of which may be related to the regulation of neuro-endocrine-immune system related biological indicators in insomnia mice.
作者
王瑞英
苏丹
李惠珍
刘亚丽
朱根华
杨明
艾志福
罗涛
薛冰
宋永贵
WANG Ruiying;SU Dan;LI Huizhen;LIU Yali;ZHU Genhua;YANG Ming;AI Zhifu;LUO Tao;XUE Bing;SONG Yonggui(Key Laboratory of Traditional Chinese Medicine for Prevention and Treatment of Brain Diseases with Cognitive Impairment/Key Research Room for Chinese Medicine Efficacy Evaluation(Prevention and Treatment of Mental Disorders Brain Disease)of Jiangxi Traditional Chinese Medicine Administration/Key Research Room for Depression Syndrome Animal Models of Jiangxi Traditional Chinese Medicine Administration,Jiangxi University of Chinese Medicine,Nanchang 330004,China;Key Laboratory of Efficacy and Safety Evaluation of Jiangxi Health Commission/Key Laboratory of Efficacy and Quality Evaluation of Anti-inflammatory Traditional Chinese Medicine of Jiangxi Administration of Traditional Chinese Medicine,Nanchang Medical College,Nanchang 330004,China;Jiangxi Guxiang Jinyun Comprehensive Health Industry Co.,Ltd.,Nanchang 330029,China;Blood Purification Center,the First Affiliated Hospital of Nanchang University,Nanchang 330004,China)
出处
《中国药房》
CAS
北大核心
2023年第5期525-530,536,共7页
China Pharmacy
基金
国家自然科学基金资助项目(No.82260764)
江西省科技计划项目(No.20212BAB206012)
江西省中医药科技计划项目(No.2020Z004)
江西省中医药科技计划重点项目(No.2020Z003)。
关键词
生五味子
酒五味子
神经-内分泌-免疫网络
失眠
raw Schisandra chinensis
wine-processed Schisandra chinensis
neuro-endocrine-immune network
insomnia
