摘要
目的基于生物信息学的方法研究NDC1基因用于肝癌的诊断及预后评估方面的作用。方法以TCGA数据库和GTEx为发现数据库,使用软件下载所有肝癌患者以及健康人的level 3 HTSeq-TPM格式的RNA测序结果,绘制箱式图及点图用于统计NDC1基因在肝癌组织及正常组织中的差异化表达情况,并使用GEPIA作为验证数据库进行佐证。绘制受试者曲线来评估NDC1基因用于诊断肝癌的效能。根据NDC1基因表达量的中位数将患者区分为NDC1高表达和低表达两组,研究肝癌患者的临床病理特征以及预后情况和NDC1基因表达的关系。绘制生存曲线,探究NDC1基因的高低表达与LIHC患者的生存情况的关系。结果肝癌患者的NDC1的表达量为2.16(1.746~2.64),明显大于正常组织中的1.449(1.263~1.644),差异有统计学意义(P<0.05);同样的,在55对配对组织中,NDC1基因在肝癌组织中的表达量3.47(3.028~3.749)也明显高于对应的癌旁正常组织中NDC1的表达量2.426(2.278~2.611),差异有统计学意义(P<0.05)。受试者曲线的结果显示了NDC1用于诊断肝癌有着较高的敏感性[曲线下面积=0.843,95%置信区间为(0.809,0.877)];在针对NDC1基因表达和肝癌患者临床病理特征以及预后的关系研究中发现NDC1高表达的肝癌患者相比NDC1低表达患者有着更晚的T分期(P<0.05)和病理分期(P<0.05);此外,NDC1基因高表达的肝癌患者总体生存期也短于NDC1基因低表达的患者。结论NDC1基因有潜力成为用于肝癌诊断和预后评估的新型生物学标志物。
Objective To investigate the role of the NDC1 gene for the diagnosis and prognostic evaluation of liver hepatocellular carcinoma(LIHC) based on bioinformatics approach.Methods The TCGA and GTEx database were used as the discovery databases and RNA sequencing results in level 3 HTSeq-TPM format from all LIHC patients as well as healthy individuals were downloaded using R software, and box plots and dot plots were peformed for analyzing the differential expression of NDC1 gene in LIHC tissues and normal tissues, and GEPIA was used as a validation database for corroboration.Secondly, Receiver Operating Characteristic(ROC)curve was drawn to assess the efficacy of the NDC1 gene for the diagnosis of LIHC.Patients were divided into low NDC1 expression group and high NDC1 expression group based on the median NDC1 gene expression, and the relationship between clinicopathological characteristics and prognosis of patients with LIHC and NDC1 gene expression was investigated.The relationship between the high and low expression of NDC1 gene was explored by drawing the survival curve of LIHC patients.Results The expression of NDC1 in patients with LIHC was 2.16(1.746-2.64),which was significantly higher than that of 1.449(1.263-1.644)in normal tissues, and the difference was statistically significant(P<0.05);similarly, in 55 paired tissues, the expression of NDC1 gene in LIHC tissues was 3.47(3.028-3.749),which was also significantly higher than that in the adjacent normal tissues 2.426(2.278-2.611),with statistically significant difference(P<0.05).The results of ROC curves showed a high sensitivity of NDC1 for the diagnosis of LIHC[area under curve=0.843,95% confidence interval(0.809,0.877)];In the study of the relationship between NDC1 gene expression and the clinicopathological characteristics and prognosis of LIHC patients, we found that the LIHC patients with high NDC1 expression had more advanced T-stage and pathological stage compared with those with low NDC1 expression(P<0.05);moreover, the overall survival of LIHC patients with high NDC1 gene expression was shorter than that of patients with low NDC1 gene expression.Conclusion The NDC1 gene has the potential to be a novel biological marker for the diagnosis and prognostic evaluation of LIHC.
作者
吴嘉旭
胡明超
王强
俞文渊
许华
WU Jiaxu;HU Mingchao;WANG Qiang;YU Wenyuan;XU HUA(Shengze School of Clinical Medicine,Kangda College of Nanjing Medical University,Department of General Surgery,the Affiliated Jiangsu Shengze Hospital of NanG jing Medical University,Suzhou,Jiangsu 215228,China)
出处
《福建医药杂志》
CAS
2023年第1期7-12,共6页
Fujian Medical Journal
基金
苏州市医学重点学科(SZXK202126)
南京医科大学康达学院科研发展基金(KD2022KYJJZD015)。
关键词
肝癌
NDC1基因
预后
生物信息学
liver hepatocellular carcinoma
NDC1 gene
prognosis
bioinformatics
