摘要
荧光传感器具有灵敏度高、响应快、操作简便等优点;然而,在使用单发射荧光传感器时,背景信号、温度、p H值等因素都会对检测结果产生干扰.为使检测结果更灵敏、准确,具有自校正特性的比率荧光传感器应运而生.将模拟抗原抗体特异性结合作用的分子印迹技术引入到比率荧光体系中,即分子印迹比率荧光(molecular imprinting ratiometric fluorescence,MI-RFL)传感器,具有高选择、高灵敏及便捷性等特点,广受关注.本文梳理了2017年以来MI-RFL传感器构建与应用的研究进展.首先介绍了MI-RFL传感器的荧光源和工作机理,然后重点综述了能够提高传感器性能的印迹策略、荧光双/三发射类型,讨论了传感器的微型设计及在现场检测中的应用,最后,尝试提出了MI-RFL传感器面临的挑战,并展望了其发展前景.
Fluorescence sensors possess excellent advantages of high sensitivity,rapid response and simple operation.However,for single-emission fluorescence sensors,some uncontrollable factors,such as background signal,temperature,and p H,can interfere with the detection results.In order to attain more sensitive and accurate detection results,ratiometric fluorescence sensors come into being,with self-correcting characteristics.Molecular imprinting technology(MIT)that mimics the specific binding effect of antigen and antibody is introduced into the ratiometric fluorescence system,and thereby constitutes the molecular imprinting-based ratiometric fluorescence(MI-RFL)sensors.The sensors have the excellent performances such as high selectivity,high sensitivity and convenience,and receive wide concerns.Herein,we review the research advances of construction and application of MI-RFL sensors since 2017.Firstly,the fluorescence source and working mechanism of MI-RFL sensor are introduced.Then,the preparation strategy of imprinting material and fluorescence double/triple emission types developed to improve the performance of the MI-RFL sensors are highlighted.In addition,the micromation design of the MI-RFL sensor and application in field detection are discussed.Finally,the challenges of MI-RFL sensors and their promising future are proposed.
作者
温宇浩
孙大妮
于佳洛
齐骥
张忠
宋志花
王晓艳
刘惠涛
陈令新
李金花
Yuhao Wen;Dani Sun;Jialuo Yu;Ji Qi;Zhong Zhang;Zhihua Song;Xiaoyan Wang;Huitao Liu;Lingxin Chen;Jinhua Li(CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation,Shandong Key Laboratory of Coastal Environmental Processes,Shandong Research Center for Coastal Environmental Engineering and Technology,Yantai Institute of Coastal Zone Research,Chinese Academy of Sciences,Yantai 264003,China;School of Marine Science and Technology,Harbin Institute of Technology(Weihai),Weihai 264209,China;College of Chemistry and Chemical Engineering,Yantai University,Yantai 264005,China;Shaanxi Engineering Laboratory for Food Green Processing and Safety Control,College of Food Engineering and Nutritional Science,Shaanxi Normal University,Xi'an 710062,China;Key Laboratory of Molecular Pharmacology and Drug Evaluation,Ministry of Education,Shandong Provincial High-eficiency Collaborative Innovation Center for Novel Formulations and Biotechnology Drug Research,School of Pharmacy,Yantai University,Yantai 264005,China;School of Pharmacy,Binzhou Medical College,Yantai 264003,China)
出处
《中国科学：化学》
CAS
CSCD
北大核心
2023年第2期196-206,共11页
SCIENTIA SINICA Chimica
基金
国家自然科学基金(编号:22176210,22106179,21876199,21976209)
山东省自然科学基金(编号:ZR2020KC032,ZR2020QB105)
烟台市科技创新发展计划(编号:2020MSGY112,2020MSGY077)
陕西师范大学攀登学者项目(编号:GK202206038)
泰山学者特聘专家项目(编号:ts20190962)资助。
关键词
比率荧光
传感器
分子印迹
构建
应用
ratiometric fluorescence
sensors
molecular imprinting
construction
application
