摘要
目的分析甘蓝型油菜的过氧化物酶(Peroxiredoxin,PRDX)家族成员的生物学信息,以期为油菜及相近植物中PRDX的酶学特征及植物抗氧化的分子机制研究提供依据.方法利用生物信息学方法,分析NCBI(National Center for biotechnology information)数据库中注册的7种油菜PRDX蛋白质的理化性质、信号肽、磷酸化位点、糖基化位点、蛋白质二级结构、三级结构及系统进化特点.结果甘蓝型油菜PRDX蛋白质比较稳定;氨基酸序列中无信号肽,且不是分泌蛋白;该家族蛋白质中存在较多潜在糖基化位点和磷酸化位点;蛋白质二级结构、三级结构预测结果显示其结构具有极大相似性,且主要以α-螺旋和无规则卷曲为主;系统进化树将该家族成员分为了两个亚家族.结论本研究为进一步探索植物PRDX家族的生物学功能提供了参考依据.
Objective The members of Peroxiredoxin(PRDX)family in Brassica napus were analyzed in order to provide the basis for studying the enzymatic characteristics of PRDX in rape and similar plants and the molecular mechanism of plant antioxidant.Method The physical and chemical properties,signal peptides,phosphorylation sites,glycosylation sites,secondary structure,tertiary structure and phylogenetic characteristics of 7 kinds of rapeseed PRDX protein registered in NCBI(National Center for biotechnology information)database were analyzed by bioinformatics methods.Results The protein of Brassica napus was relatively stable.There is no signal peptide in the amino acid sequence,and it is not a secretory protein;There are many potential glycosylation sites and phosphorylation sites in rapeseed PRDX.The prediction results of secondary structure and tertiary structure of protein show that their structures are very similar,and mainlyα-helix and random curl;Phylogenetic tree divided the family members into two subfamilies.Conclusion This study provides a reference for further exploring the biological functions of PRDX family.
作者
林睿
叶思萍
李雨澎
杨文闯
郭笑
LIN Rui;YE Siping;LI Yupeng;YANG Wenchuang;GUO Xiao(Pharmacy College of Beihua University,Jilin 132013,China;Basic Medical College of Beihua University,Jilin 132013,China)
出处
《北华大学学报（自然科学版）》
CAS
2023年第1期58-63,共6页
Journal of Beihua University（Natural Science）
基金
国家自然科学基金青年基金项目(3900918)
吉林省卫生与健康创新项目资助课题(2020J024)
北华大学研究生创新项目(2021022)
北华大学大学生创新计划项目(202110201090)。
