摘要
目的 分析细胞分离液Percoll分离法和十二烷基硫酸钠(SDS)沉淀法联合基质辅助激光解析电离飞行时间质谱(MALDI-TOF MS)在快速鉴定血流感染病原菌中的应用。方法 收集2022年1—11月齐齐哈尔医学院附属第三医院检测的300份血培养报警阳性标本(报警时间<48 h),通过Percoll分离法和SDS沉淀法快速提取血培养阳性病原菌,并采用MALDI-TOF MS法直接鉴定提取物,将鉴定结果与传统血培养16~24 h的结果进行比较。结果 300份血液标本分离出210株革兰阴性(G-)菌、78株革兰阳性(G+)菌、12株念珠菌。采用Percoll分离法鉴定G+菌、G-菌、念珠菌的鉴定率均明显高于SDS沉淀法(G+菌:92.31%比76.92%,G-菌:98.57%比90.48%,念珠菌:83.33%比33.33%,均P<0.05)。MALDI-TOF MS对Percoll分离法处理后的G+菌、G-菌鉴定率均明显高于SDS沉淀法处理后的病原菌鉴定率(G+菌:89.74%比64.10%,G-菌:95.71%比80.96%,均P<0.05)。MALDI-TOF MS对Percoll分离法处理后标本病原菌鉴定率与传统血培养真菌鉴定率比较差异无统计学意义。MALDI-TOF MS对SDS沉淀法处理后的G+菌、G-菌鉴定率均明显低于传统血培养。结论 Percoll分离法联合MALDI-TOF MS可提高血流感染病原菌的鉴定率,适用于直接鉴定病原菌,与传统血培养鉴定结果接近,具有成本低廉、操作简单等优点,值得推广。
Objective To analyze the application of cell separation solution Percoll seperation and sodium dodecyl sulfate(SDS) precipitation combined with matrix assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF MS) in rapid identification of pathogens of bloodstream infection. Methods The 300 positive blood culture samples(alarm time < 48 hours) were collected from the Third Affiliated Hospital of Qiqihar Medical College from January 2022 to November 2022. Percoll separation method and SDS precipitation method were used to quickly extract the positive pathogens in blood culture, and then MALDI-TOF MS method was used to directly identify the extracts. The results of identification were compared with those of traditional blood culture for 16-24 hours. Results The 210 strains of Gram negative(G-) bacteria, 78 strains of Gram positive(G+)bacteria and 12 strains of Candida were isolated from 300 blood samples. The identification rates of G+bacteria,G-bacteria and Candida were significantly higher by Percoll separation than those by SDS precipitation(G+bacteria: 92.31% vs. 76.92%, G-bacteria: 98.57% vs. 90.48%, Candida: 83.33% vs. 33.33%, all P < 0.05). The identification rates of G+and G-bacteria by MALDI-TOF MS after Percoll separation were significantly higher than those after SDS precipitation(G+bacteria: 89.74% vs. 64.10%,G-bacteria: 95.71% vs. 80.96%, both P < 0.05).There was no statistical difference between the identification rate of pathogenic bacteria in the samples treated with Percoll seperation method by MALDI-TOF MS and that of traditional blood culture. The identification rates of G+and G-bacteria after SDS precipitation were lower than those by traditional blood culture. Conclusions Percoll separation combined with MALDI-TOF MS could improve the rapid identification rate of bloodstream infection, and is suitable for direct identification of pathogenic bacteria. The results are close to those by traditional blood culture, and have the advantages of low cost and simple operation, so it is worth promoting.
作者
刘沫然
郑玉婷
李红柳
肖铟
杜欣
才奇博
Liu Moran;Zheng Yuting;Li Hongliu;Xiao Yin;Du Xin;Cai Qibo(Department of Clinical Laboratory,the Third Affiliated Hospital of Qiqihar Medical College,Qiqihar 161000,Heilongjiang,China;Department of Oncology,the Third Affiliated Hospital of Qiqihar Medical College,Qiqihar 161000,Heilongjiang,China)
出处
《实用检验医师杂志》
2022年第4期349-352,共4页
Chinese Journal of Clinical Pathologist
基金
黑龙江省齐齐哈尔市科技计划联合引导项目(LSFGG-2022014)。
关键词
细胞分离法
十二烷基硫酸钠沉淀法
基质辅助激光解析电离飞行时间质谱
血流感染
Cell separation
Sodium dodecyl sulfate precipitation method
Matrix assisted laser desorption ionization time-of-flight mass spectrometry
Bloodstream infection
