摘要
为了确定辽宁绒山羊毛尾线虫的种类及遗传进化关系,以来自辽宁省东部山区的10株辽宁绒山羊源毛尾线虫为研究对象,采用PCR技术对其核糖体基因组内转录间隔区(ITS)进行扩增并测序,然后将测序结果与GenBank中公布的其他动物毛尾线虫进行同源性分析并构建系统进化树。结果显示:10株虫体的形态结构均符合毛尾线虫特征,10株毛尾线虫的ITS1序列长度基本一致,约为445 bp;与GenBank中登录的2株中国岩羊毛尾线虫同源性最高,分别为95.5%~100%和93.7%~98.7%;系统进化树分析显示,10株绒山羊毛尾线虫样品与2株中国岩羊毛尾线虫聚集在同一分支上,种内差异(0~2.1%)远小于种间差异(23.6~50.6%)。结果表明,本次从辽宁绒山羊体内分离到的线虫为毛尾线虫,同时也证实其核糖体ITS序列种内相对保守,可以作为毛尾线虫的分子鉴定及遗传进化研究的目的基因。
In order to identify the species and genetic evolutionary relationship of Trichuris in Liaoning cashmere goats,10 strains of Trichuris derived from the goats from eastern mountainous areas in the province were studied,their internal transcribed spacer(ITS)was amplified by PCR and then sequenced,followed by homology analysis on the results and other animal Trichuris published in GenBank,then construction of a phylogenetic tree.The results showed that the morphology and structure of the 10 strains conformed to the characteristics of Trichuris,and their ITS1 sequence length was basically consistent,which was about 445 bp;the homology with the two stains of Trichuris in Chinese blue sheep registered in GenBank was highest,from 95.5%to 100%,and from 93.7%to 98.7%,respectively;it was shown that,by phylogenetic tree analysis,all the samples were clustered in the same branch as the above two strains,and the intraspectic difference(from 0 to 2.1%)was much smaller than the interspecific one(from 23.6%to 50.6%).In conclusion,the Trichuris isolated from Liaoning cashmere goats belonged to Trichuris ovis,the ribosomal ITS sequence was relatively conservative within species,which might be used as the target gene for molecular identification and genetic evolution research of Trichuris.
作者
郑毓秀
于本良
王大为
刘宇明
李冰
刘孝刚
Zheng Yuxiu;Yu Benliang;Wang Dawei;Liu Yuming;Li Bing;Liu Xiaogang(College of Animal Husbandry and Veterinary Medicine,Jinzhou Medical University,Jinzhou,Liaoning 121000,China;Liaoning Provincial Center for Animal Disease Control and Prevention,Shenyang,Liaoning 110164,China)
出处
《中国动物检疫》
CAS
2023年第3期20-24,共5页
China Animal Health Inspection
基金
辽宁省科学技术厅农业攻关及产业化项目(2015103036)。
关键词
辽宁绒山羊
毛尾线虫
分子鉴定
遗传进化分析
Liaoning cashmere goat
Trichuris
molecular identification
genetic evolutionary analysis
