摘要
背景:类风湿关节炎是常见的自身免疫性疾病,主要侵犯机体关节部位,特征为持续性滑膜炎症,研究提示促炎性的细胞焦亡与类风湿关节炎疾病的发生发展密切相关。目的:明确类风湿关节炎患者血清和关节液中细胞焦亡相关因子NOD样受体蛋白3、半胱氨酸天冬氨酸蛋白酶1、白细胞介素18及白细胞介素1β的表达水平及其临床意义。方法:选择2021年1月至2022年1月河南省洛阳正骨医院(河南省骨科医院)风湿病科收治的类风湿关节炎患者24例(类风湿关节炎组),同期选取伴膝关节积液的骨关节炎患者30例(骨关节炎组),健康受试者40例(对照组)。检测并记录3组受试者的C-反应蛋白、红细胞沉降率、抗环瓜氨酸肽抗体、类风湿因子水平和类风湿关节炎28个关节疾病活动度评分。采用酶联免疫吸附法测定血清和关节液中NOD样受体蛋白3、半胱氨酸天冬氨酸蛋白酶1、白细胞介素18及白细胞介素1β的表达水平,分析上述4项因子表达水平与类风湿关节炎患者临床指标之间的相关性。结果与结论:①类风湿关节炎组患者血清中的NOD样受体蛋白3、半胱氨酸天冬氨酸蛋白酶1、白细胞介素18及白细胞介素1β水平显著高于对照组(P<0.0001),关节液中NOD样受体蛋白3、半胱氨酸天冬氨酸蛋白酶1、白细胞介素18及白细胞介素1β水平显著高于骨关节炎组(P<0.05-0.01);②类风湿关节炎患者血清和关节液中NOD样受体蛋白3、半胱氨酸天冬氨酸蛋白酶1、白细胞介素18及白细胞介素1β的表达水平分别与类风湿关节炎患者的类风湿关节炎28个关节疾病活动度评分、C-反应蛋白和红细胞沉降率水平呈正相关;③提示细胞焦亡相关因子在类风湿关节炎患者中明显升高,且与疾病活动度相关,提示细胞焦亡可能在类风湿关节炎发病中发挥作用。
BACKGROUND:Rheumatoid arthritis is a common autoimmune disease that mainly affects the joints and is characterized by persistent synovial inflammation.Studies have suggested that proinflammatory pyroptosis is closely related to the occurrence and development of rheumatoid arthritis.OBJECTIVE:To investigate the expression levels of pyroptosis-related factors NOD-like receptor protein 3,cysteine aspartic acid-specific protease-1(caspase-1),interleukin-18 and interleukin-1βin serum and articular fluid of patients with rheumatoid arthritis and their clinical significance.METHODS:From January 2021 to January 2022,24 patients with rheumatoid arthritis admitted to the Rheumatology Department of Luoyang Orthopedic-Traumatological Hospital of Henan Province(Henan Provincial Orthopedic Hospital)were selected as rheumatoid arthritis group.Thirty osteoarthritis patients with knee joint effusion were selected as osteoarthritis group.Forty healthy subjects were selected as control group.C-reactive protein level,erythrocyte sedimentation rate,anti-cyclic citrullinated peptide antibody level,rheumatoid factor levels,and disease activity score in 28 joints were measured and recorded.NOD-like receptor protein 3,caspase-1,interleukin-18 and interleukin-1βlevels in serum and joint fluid were determined by enzyme-linked immunosorbent assay(ELISA)to analyze the correlation between their expression levels and the clinical indicators of patients with rheumatoid arthritis.RESULTS AND CONCLUSION:The serum levels of NOD-like receptor protein 3,caspase-1,interleukin-18 and interleukin-1βin the rheumatoid arthritis group were significantly higher than those in the control group(P<0.0001),while the articular fluid levels of NOD-like receptor protein 3,caspase-1,interleukin-18 and interleukin-1βin the rheumatoid arthritis group were significantly higher than those in the osteoarthritis group(P<0.05-0.01).The expression levels of NOD-like receptor protein 3,caspase-1,interleukin-18 and interleukin-1βin serum and articular fluid of patients with rheumatoid arthritis were positively correlated with D disease activity score in 28 joints,C-reactive protein level,and erythrocyte sedimentation rate.To conclude,pyroptosis-related factors are significantly increased in patients with rheumatoid arthritis and correlated with disease activity,suggesting that pyroptosis may play a role in the pathogenesis of rheumatoid arthritis.
作者
王秋苑
刘又文
岳辰
侯宏理
张雪
Wang Qiuyuan;Liu Youwen;Yue Chen;Hou Hongli;Zhang Xue(Henan University of Chinese Medicine,Zhengzhou 450046,Henan Province,China;Luoyang Orthopedic-Traumatological Hospital of Henan Province(HenanProvincial Orthopedic Hospital),Luoyang 471000,Henan Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2023年第32期5209-5213,共5页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金(82004396),项目负责人:张雪
河南省科技攻关(212102311089),项目负责人:张雪。
关键词
类风湿关节炎
细胞焦亡
血清
关节液
炎性因子
疾病活动度
rheumatoid arthritis
pyroptosis
serum
joint fluid
inflammatory cytokine
disease activity
