摘要
采用气相色谱三重四极杆质谱(GC-MS/MS)技术,研究了5种苯系物暴露后在贻贝各组织中的分布、蓄积和消除规律.结果表明,贻贝不同组织中对5种苯系物的生物蓄积具有一定的差异,且分布呈组织特异性.暴露试验结束后,贻贝各组织中5种苯系物分布及其含量变化趋势基本相同,消化腺和性腺中的苯系物含量增加比其他组织快,且消化腺和性腺中的峰值比其他4种组织中的峰值较高.消化腺和性腺中的苯系物含量相对较高,有明显的吸收和富集作用.足、鳃、闭壳肌和外套膜中5种苯系物的含量相对较低,约为消化腺中的20%左右.消除阶段贻贝各组织中5种苯系物的含量符合一阶非线性衰减模型,相关系数范围为0.9948~0.9999.暴露组净化1 d后,贻贝对苯、甲苯、间二甲苯、对二甲苯和邻二甲苯的净化率范围分别为98.4%~99.2%、97.3%~98.8%、86.1%~95.8%、90.8%~97.0%和92.1%~96.6%.净化5 d后,暴露组贻贝各组织中苯系物的含量虽趋于空白组水平,但仍有一定的残留.
The distribution,accumulation and elimination of 5 benzene series in mussel tissues were studied using the gas chromatography tandem mass spectrometry(GC-MS/MS).The results showed that there were some differences in the bioaccumulation of 5 benzene series in different tissues of mussel,and the distribution was tissue specific.At the end of the exposure experiment,the distribution and the trend of variation in content the five benzene series in the six tissues of mussels were basically the same.The content of benzene series in digestive glands and gonads increased faster than in other tissues,and the peaks values in the digestive gland and gonad were higher than those in the other four tissues.The contents of benzene series in the digestive gland and gonad were relatively higher,which had obvious absorption and enrichment effect.The contents of 5 benzene series in foot,gill,adductor and mantle were relatively lower,about 20% of that in the digestive gland.The contents of 5 benzene series in mussel tissues during elimination stage were consistent with the first-order nonlinear attenuation model,with correlation coefficients ranging from 0.9948 to 0.9999.The purification rates of benzene,toluene,m-xylene,p-xylene and o-xylene of mussels were 98.4%~99.2%,97.3%~98.8%,86.1%~95.8%,90.8%~97.0%and 92.1%~96.6%respectively after 1 day of purification in the exposed group.After 5 days of purification,although the content of benzene series in the tissues of the exposed group tended to be at the level of blank group,but there were still some residues.
作者
晁铎源
宋立玲
王红光
丁顒
邵宏宏
周秀锦
CHAO Duoyuan;SONG Liing;WANG Hongguang;DING Yong;SHAO Honghong;ZHOU Xiujin(Zhoushan Customs Comprehensive Technical Service Center,Zhoushan,316000,Zhejiang China;Zhejiang Marine Ecology and Environment Monitoring Center,Zhoushan,316000,Zhejiang China)
出处
《分析测试技术与仪器》
CAS
2023年第1期68-75,共8页
Analysis and Testing Technology and Instruments
基金
浙江省基础公益项目(LGN19C190002)
舟山市公益项目(2019C31045)。
