摘要
目的:建立HPLC法测定越鞠保和丸中苍术素、木香烃内酯和去氢木香内酯的含量。方法:使用C18(250 mm×4.6 mm,5μm)色谱柱,以甲醇作为流动相A,0.2%磷酸水溶液作为流动相B,梯度洗脱;检测波长为木香烃内酯、去氢木香内酯225 nm,苍术素340 nm;柱温40℃;流速1.0 ml/min;进样量为10μl。结果:苍术素在1.036 79~103.679μg/ml范围内线性良好(r=1.000 0),木香烃内酯在2.951 17~118.046 8μg/ml范围内线性良好(r=1.000 0),去氢木香内酯在3.034~303.4μg/ml范围内线性良好(r=1.000 0)。苍术素平均回收率为103.20%,RSD为0.84%;木香烃内酯平均回收率为103.06%,RSD为0.52%;去氢木香内酯平均回收率为91.80%,RSD为0.72%。结论:本方法操作简单,专属性强,可以用于越鞠保和丸中苍术与木香两味药材的质量控制。
Objective:To establish an HPLC method for the determination of atractylodin,costunolide and dehydrocostuslactone in Yuejubaohe pills.Methods:C18(250 mm×4.6 mm,5μm)chromatographic column was used,with methanol as mobile phase A and 0.2%phosphoric acid aqueous solution as mobile phase B,gradient elution;Detection wavelengths for costunolide and dehydrocostuslactone was 225 nm,for atractylodin was 340 nm;Column temperature was 40℃;Flow rate was 1 ml/min;Injection volume was 10μl.Results:The linearity was good in the range of 1.03679~103.679μg/ml for atractylodin(r=1.0000),2.95117~118.0468μg/ml for costunolide(r=1.0000)and 3.034~303.4μg/ml for dehydrocostuslactone(r=1.0000).The average recovery rate of atractylodin was 103.20%with the RSD was 0.84%;the average recovery rate of costunolide was 103.06%with the RSD was 0.52%;the average recovery rate of dehydrocostuslactone was 91.80%with the RSD was 0.72%.Conclusion:This method is simple to operate and has strong specificity,can be used for the quality control of atractylodes and aucklandiae radix in Yuejubaohe Pills.
作者
安劼
牛辰瑾
曲佳
申琳
An Jie;Niu Chenjin;Qu Jia;Shen Lin(Wuqing Branch of Tianjin Institute of Drug Control,Tianjin 301700;Tianjin Institute for Drug Control,Tianjin 300070;Second Teaching Hospital of Tjanjin University of Traditional Chinese Medcine,Tianjin 300250)
出处
《天津药学》
2023年第1期17-21,共5页
Tianjin Pharmacy
基金
国家中医药管理局全国中药特色技术传承人才项目(No.T20194828003)。
