摘要
目的 探讨纵隔肿瘤患者术后肺部感染与转化生长因子β1(TGF-β1)、白细胞介素(IL)-6及肿瘤坏死因子-α(TNF-α)基因多态性的关系。方法 选择2016年3月至2022年3月在江西中医药大学附属医院接受纵隔肿瘤切除术后发生肺部感染患者32例(感染组)、同期接受纵隔肿瘤切除术后未发生肺部感染患者30例(对照组)为研究对象,分析TGF-β1、IL-6及TNF-α基因的多态性。结果 感染组血清炎性细胞因子TGF-β1、IL-6以及TNF-α水平均高于对照组,差异均有统计学意义(P<0.05)。TGF-β1基因rs10417924位点基因型为CC、CT、TT,rs4803455位点基因型为CC、CT、TT;两组rs10417924位点基因型和等位基因分布频率比较,差异均有统计学意义(P<0.05),两组rs4803455位点基因型和等位基因分布频率比较,差异均无统计学意义(P>0.05),其中rs10417924位点T等位基因的OR为1.385(95%CI 1.021~1.764)。IL-6基因rs1800796位点基因型为CC、GC、GG,rs2069837位点基因型为AA、GA、GG;两组rs1800796、rs2069837位点基因型和等位基因分布频率比较,差异均无统计学意义(P>0.05)。TNF-α基因rs1800629位点基因型为GG、GA、AA,rs1799724位点基因型为GG、GA、AA;两组rs1800629、rs1799724位点基因型和等位基因分布频率比较,差异均有统计学意义(P<0.05)。其中rs1800629位点A等位基因的OR为1.315(95%CI 1.044~1.653),rs1799724位点A等位基因的OR为1.651(95%CI 1.211~2.584)。3项指标单独检测的曲线下面积(AUC)均小于联合检测的AUC(0.870),联合检查的灵敏度为0.848,特异度为0.774,临界值为0.622 ng/L。结论 血清TGF-β1、IL-6及TNF-α可用于纵隔肿瘤患者术后肺部感染的诊断,且TGF-β1基因rs10417924位点T等位基因、TNF-α基因rs1800629、rs1799724位点A等位基因与患者术后肺部感染有关。
Objective To investigate the relationship between postoperative lung infection with the polymorphisms of transforming growth factor-β1(TGF-β1),interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)genes in the patients with mediastinal tumors.Methods Thirty-two patients with lung infection after mediastinal tumor resection(infection group)in this hospital from March 2016 to March 2022 and 30 patients with mediastinal tumor without postoperative lung infection during the same period(control group)were selected as the research subjects.The polymorphisms of TGF-β1,IL-6 and TNF-αgenes were analyzed.Results Serum inflammatory factor TGF-β1,IL-6 and TNF-αlevels in the infection group were higher than those in the control group,and the differences were statistically significant(P<0.05).The genotype at rs10417924 locus of TGF-β1 gene was CC,CT and TT,and the genotype at rs4803455 locus was CC,CT and TT;there was statistically significant difference in genotype and allele distribution frequency at rs10417924 locus between the two groups(P<0.05),and there was no statistically significant difference in genotype and allele distribution frequency at rs4803455 locus between the two groups(P>0.05).The OR of T allele at rs10417924 locus was 1.385(95%CI 1.021-1.764).The genotype at rs1800796 locus of IL-6 gene was CC,GC,GG,and the genotype at rs2069837 locus was AA,GA,GG;there was no statistically significant difference in the genotype and allele distribution frequency at rs1800796 locus between the two groups(P>0.05),and there was no statistically significant difference in the genotype and allele distribution frequency at rs2069837 locus between the two groups(P>0.05).The genotype at rs1800629 locus in TNF-αgene was GG,GA,AA,and the genotype at rs1799724 locus was GG,GA and AA;there was statistically significant difference in genotype and allele distribution frequency at rs1800629 locus between the two groups(P<0.05),and there was significant difference in genotype and allele distribution frequency at rs1799724 locus between the two groups(P<0.05).The OR of allele A at rs1800629 locus was 1.315(95%CI 1.044-1.653),and that of allele A at rs1799724 locus was 1.651(95%CI 1.211-2.584).The area under the curve(AUC)of the three indexes single detection was smaller than the AUC(0.870)of the joint test.The sensitivity of the joint test was 0.848,the specificity was 0.774,and the optimal cut-off value was 0.622 ng/L.Conclusion Serum TGF-β1,IL-6 and TNF-αcould be used for the diagnosis of postoperative pulmonary infection in the patients with mediastinal tumors,moreover T allele at rs10417924 locus of TGF-β1 gene,A alleles at rs1800629 and rs1799724 loci in TNF-αgene are associated with postoperative pulmonary infection.
作者
于华明
熊国江
匡仁青
赵云
YU Huaming;XIONG Guojiang;KUANG Renqing;ZHAO Yun(Department of Cardiothoracic Surgery,Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine,Nanchang,Jiangxi 330006,China;Graduate School,Jiangxi University of Traditional Chinese Medicine,Nanchang,Jiangxi 330006,China)
出处
《检验医学与临床》
CAS
2023年第7期960-963,968,共5页
Laboratory Medicine and Clinic
基金
江西省卫生健康委员会普通科技计划(2022107531)。
关键词
转化生长因子Β1
白细胞介素-6
肿瘤坏死因子-α
纵隔肿瘤
术后肺部感染
基因多态性
transforming growth factor-β1
interleukin-6
tumor necrosis factor-α
mediastinal neoplasms
postoperative pulmonary infection
genetic polymorphism
