摘要
目的博来霉素(BLM)分别诱导系统性硬化症(SSc)小鼠模型及肺纤维化小鼠模型,观察两种模型皮肤及肺的病理改变及羟脯氨酸(HYP)含量变化,为“肺主皮毛”理论提供实验依据。方法分别建立SSc小鼠模型和肺纤维化小鼠模型,两种模型分别设置注射BLM模型组和注射生理盐水对照组。取每组相应皮肤和肺脏,进行HE染色和HYP含量检测。结果系统性硬化症模型和肺纤维化模型小鼠的肺组织和表皮均出现明显病理改变,且HYP含量在两种小鼠动物模型皮肤和肺组织中均明显升高(P<0.05)。结论博来霉素诱导的系统性硬化症小鼠模型及肺纤维化小鼠模型都能够同时产生肺和皮肤病变,从中医角度分析,这是“肺主皮毛”的具体体现。
Objective To observe the pathological changes of skin and lung and the changes of hydroxyproline (HYP) content in mice with systemic sclerosis (SSc) and pulmonary fibrosis induced by bleomycin (BLM), so as to provide experimental basis for the theory that "lung dominates skin and hair". Methods SSc mice model and pulmonary fibrosis mice model were established respectively. BLM model group and normal saline control group were respectively set up for the two models. Take the corresponding skin and lungs of each group for HE staining and HYP content detection. Results There were obvious pathological changes in the lung tissue and epidermis of the mice with systemic sclerosis and pulmonary fibrosis, and the content of HYP in the skin and lung tissue of the two mouse models was significantly increased (P<0.05). Conclusion Both bleomycin induced systemic sclerosis mouse model and pulmonary fibrosis mouse model can produce lung and skin lesions at the same time. From the perspective of traditional Chinese medicine, this is the specific embodiment of "lung dominates skin and hair".
作者
康信忠
资晓飞
张艺楠
彭中娟
王根发
KANG Xinzhong;ZI Xiaofei;ZHANG Yinan;PENG Zhongjuan;WANG Genfa(The Second Affiliated Hospital of Nanchang University,Nanchang Jiangxi 330046,China)
出处
《药品评价》
CAS
2023年第1期19-22,共4页
Drug Evaluation
基金
江西省卫生计生委中医药科研课题项目(2018A388)。
