摘要
目的探究在CXC基序趋化因子配体8(CXC motif chemokine ligand 8,CXCL8)在口腔鳞癌(oral squamous cell carcinoma,OSCC)中的作用。方法实时荧光定量PCR(quantitative real-time PCR,RTqPCR)和Western印迹检测OSCC组织和细胞中CXCL8的表达;分析CXCL8表达与OSCC患者临床病理特征的相关性;细胞计数试剂盒8(cell counting kit-8,CCK-8)、克隆形成、流式细胞术、划痕实验、Transwell实验和Western印迹检测CXCL8敲低对细胞增殖、凋亡、迁移、侵袭和上皮间质转化(epithelial-mesenchymal transition,EMT)的影响。结果CXCL8在OSCC组织和细胞中的表达显著上调。CXCL8高水平与肿瘤大小、TNM分期和远处转移显著相关。CXCL8的敲低可以抑制SCC9细胞增殖、迁移、侵袭和EMT,促进细胞凋亡。结论CXCL8在OSCC中高表达,敲低其表达可抑制SCC9细胞的增殖和转移。
Objective To investigate the role of CXC motif chemokine ligand 8(CXCL8)in oral squamous cell carcinoma(OSCC).Methods The expression level of CXCL8 in OSCC tissues and cells was detected by RT-qPCR and Western blotting,the correlation between CXCL8 expression level and the clinicopathological features of OSCC patients was analyzed.The effect of CXCL8 knockdown on cell proliferation,apoptosis,migration,invasion and epithelial-mesenchymal transition(EMT)was detected by cell counting kit 8(CCK-8)method,colony formation assay,flow cytometry,wound healing assay,transwell assay and Western blotting.Results CXCL8 was significantly up-regulated in OSCC tissues and cells.The high expression level of CXCL8 was significantly correlated with tumor size,TNM staging and distant metastasis.CXCL8 knockdown can inhibit the proliferation,migration,invasion and EMT of SCC9 cells,and promote the apoptosis.Conclusion CXCL8 is up-regulated in OSCC,and the suppression of its expression can inhibit the proliferation and metastasis of SCC9 cells.
作者
邓亮亮
张先梅
黄婧
朱彬
周奇
DENG Liangliang;ZHANG Xianmei;HUANG Jing;ZHU Bin;ZHOU Qi(Center of Stomatology,the Central Hospital of Enshi Tujia and Miao Autonomous Prefecture,Enshi Tujia and Miao Autonomous Prefecture,Hubei,445000,China)
出处
《医学分子生物学杂志》
CAS
2023年第2期110-116,共7页
Journal of Medical Molecular Biology
基金
湖北省卫生健康委员会科研项目(No.WJ2021F138)。
