摘要
采用单因素结合响应面法优化黑果腺肋花楸全果、果渣原花青素的提取工艺,并以DPPH自由基、ABTS+自由基、羟自由基清除率和总还原能力评估其抗氧化能力。结果表明,黑果腺肋花楸全果和果渣原花青素最佳提取工艺条件为液料比45∶1(mL/g),乙醇体积分数50%,提取时间37 min,提取温度50℃,在此工艺条件下花楸全果和果渣原花青素得率分别为98.48、78.04 mg/g。体外抗氧化研究结果显示,黑果腺肋花楸全果、果渣原花青素具有良好的还原能力且对ABTS+自由基(IC50779.51、315.95μg/mL)、DPPH自由基(IC50406.73、156.73μg/mL)和羟自由基(IC501319.02、393.66μg/mL)均具有一定的清除能力,其中对DPPH自由基清除能力最优。综上,黑果腺肋花楸果渣中仍含有大量原花青素,且抗氧化能力优于全果原花青素,具有进一步开发的潜力和良好的再利用价值。
The single factor test was combined with response surface methodology to optimize the ultrasound-assisted extraction of proanthocyanidins from Aronia melanocarpa fruits and the marc.Their antioxidant activity was evaluated based on the ABTS+,DPPH,and hydroxyl radicals scavenging capacity and total reducing ability.The results showed that the optimal extraction conditions were as follows:liquid-to-material ratio of 45∶1(mL/g),50%ethanol,extraction for 37 min,and 50℃.At this optimal conditions,the yield of proanthocyanidins from the fruits and marc was 98.48 mg/g and 78.04 mg/g,respectively.The experiment on the in vitro antioxidant activity suggested that the proanthocyanidins from the fruits and marc had strong reducing ability and can scavenge ABTS+(IC50 of 779.51 and 315.95μg/mL),DPPH(IC50 of 406.73 and 156.73μg/mL),and hydroxyl radicals(IC50 of 1319.02 and 393.66μg/mL),particularly DPPH radicals.In summary,the marc of A.melanocarpa contains a lot of proanthocyanidins with the antioxidant capacity stronger than that from the fruit,which should be further developed and utilized.
作者
只德贤
李建颖
欧燕芳
陈星雨
ZHI De-xian;LI Jian-ying;OU Yan-fang;CHEN Xing-yu(School of Biotechnology and Food Science,National Food and Drug Experimental Teaching Demonstration Center,Tianjin University of Commerce,Tianjin 300134,China)
出处
《食品研究与开发》
CAS
北大核心
2023年第8期96-104,共9页
Food Research and Development
基金
天津市科技计划项目(19YFZCSN00010)。
关键词
黑果腺肋花楸
原花青素
超声辅助提取
果渣
抗氧化性
Aronia melanocarpa
proanthocyanidins
ultrasound-assisted extraction
marc
antioxidation
