摘要
【目的】探究AP2/ERF转录因子在绿豆干旱胁迫响应中的应答机制。【方法】基于全基因组测序数据对绿豆VrERF家族基因的种间同源性、互作蛋白功能及顺式作用元件进行分析,通过转录组测序数据分析各VrERF基因的组织特异性表达、干旱胁迫下基因表达差异情况并用q RT-PCR进行验证。【结果】绿豆与豌豆、菜豆和大豆的ERF家族基因间不仅具有相同的进化起源,还存在明显的基因扩张现象。VrERF基因启动子区均具有多个与激素响应、胁迫响应以及生长发育相关的顺式作用元件。此外,不同的绿豆VrERF蛋白间存在广泛的互作关系,可能通过与b ZIP、RAP2.4和STZ等蛋白互作参与绿豆非生物胁迫的防御响应。基因表达分析显示,多数VrERF家族基因的表达具有较强的组织特异性,且在干旱胁迫下,VrERF7/62在绿豆VC1973A和JP226873叶片中均显著下调表达,qRTPCR检测也显示VrERF7/62的表达受干旱胁迫诱导显著下调。【结论】推测VrERF7/62可能在绿豆干旱胁迫响应过程中起负调控作用,结果也为绿豆AP2/ERF家族基因的抗逆性表达及基因功能研究奠定了基础。
【Objective】 In order to explore the response mechanism of AP2/ERF transcription factor in mung bean under drought stress.【Method】 The interspecies homology,interacting protein function and cis-acting elements of VrERF family genes in mung bean were analyzed based on the whole genome sequencing data.The tissue-specific expression of each VrERF gene and the difference of gene expression under drought stress were analyzed by transcriptome sequencing data and verified by qRT-PCR.【Result】 The ERF family gene of mung bean had that same evolutionary origin as those of pea,kidney bean and soybean,and also had obvious gene expansion.The promoter region of VrERF gene has many cis-acting elements related to hormone response,stress response and growth.In addition,there are extensive interactions among different VrERF proteins in mung bean,which may participate in the defense response of mung bean to abiotic stress through interaction with bZIP,RAP2.4 and STZ.Gene expression analysis shows that the expression of most VrERF family genes has strong tissue specificity,and VrERF7/62 is significantly down-regulated in mung bean VC1973A and JP226873 leaves under drought stress.q RT-PCR analysis also showed that the expression of VrERF7/62 was significantly down-regulated by drought stress.【Conclusion】 This study speculated that VrERF7/62 might play a negative role in the response to drought stress in mung bean,and laid a foundation for the study of the expression and gene function of AP2/ERF family genes in mung bean.
作者
李蒙鑫
丁冬会
张雨朋
景琳超
鲁建荣
刘嘉斐
陈吉宝
杨树琼
LI Mengxin;DING Donghui;ZHANG Yupeng;JING Linchao;LU Jianrong;LIU Jiafei;CHEN Jibao;YANG Shuqiong(Key Laboratory of Ecological Security for Water Source Region of Mid-Line of South-to-North Diversion Project of Henan Province,Nanyang 473061,Henan,China;Henan Key Laboratory of Insect Biology in Funiu Mountain,Nanyang 473061,Henan,China)
出处
《四川农业大学学报》
CSCD
北大核心
2023年第2期287-294,334,共9页
Journal of Sichuan Agricultural University
基金
河南省科技攻关专项(222102110275)
南阳师范学院博士科研专项(2019ZX027)
南阳师范学院研究生创新基金项目(2021CX010)
河南省高等学校大学生创新训练计划项目(202210481023)。
