摘要
采用20 L发酵罐平台对饲用凝结芽孢杆菌W-02菌株进行分批发酵,实时监测发酵液的溶氧含量、吸光度、pH值、活菌数和可溶性总糖含量,在此基础上采用Logistic方程和Luedeking-Piret方程分别拟合了菌体生长和可溶性总糖消耗的动力学方程,获得了该菌株分批发酵的动力学方程模型和参数。结果表明:菌体生长模型为X=X_(0)X_(max)e^(μ_(max)t)/X_(max)-X_(0)+X_(0)e^(μ_(max)t)=27.7459e^(0.2524t)/58.53+0.47e^(0.2524t);可溶性总糖消耗模型为S=10.65538-3.26742e^(0.2524t)/58.7+0.3e^(0.2524t)-0.13561n(0.9949+0.0051e^(0.2524t));2个模型计算的模拟值与验证试验的试验值拟合度均较好,平均相对误差均小于10%。该结果为凝结芽孢杆菌实际生产中发酵过程自动化监测和实时控制提供了依据。
Bacillus coagulans strain W-02 was fermented in batches in 20 L fermentor.During the fermentation process,the dissolved oxygen,absorbance,pH value,viable bacterium count,and soluble total sugar content were measured in real time.Thereafter,the Logistic equation and Luedeking-Piret equation were employed to respectively fit the dynamics of bacterium growth and soluble total sugar consumption.The kinetic models and parameters describing the batch fermentation process were obtained.The results showed that the bacterial growth model was X=X_(0)X_(max)e^(μ_(max)t)/X_(max)-X_(0)+X_(0)e^(μ_(max)t)=27.7459e^(0.2524t)/58.53+0.47e^(0.2524t),and the model of soluble total sugar consumption was S=10.65538-3.26742e^(0.2524t)/58.7+0.3e^(0.2524t)-0.13561n(0.9949+0.0051e^(0.2524t)).The analogue values calculated from the obtained two kinetic equations were highly in fit with the experimental values,with average relative errors of less than 10%.The results provide a basis for automatic monitoring and real-time control of fermentation process in actual production of Bacillus coagulans.
作者
曾发姣
解丽娟
王升平
程刚毅
曾奥
舒燕
周映华
高书锋
ZENG Fa-jiao;XIE Li-juan;WANG Sheng-ping;CHENG Gang-yi;ZENG Ao;SHU Yan;ZHOU Ying-hua;GAO Shu-feng(Hunan Institute of Microbiology,Feeding Probiotics Engineering Laboratory of Hunan Province,Hunan Provincial Engineering and Technology Research Center for Agricultural Microbiology Application,Changsha 410009,PRC)
出处
《湖南农业科学》
2023年第3期1-5,共5页
Hunan Agricultural Sciences
基金
湖南省自然科学基金(2017JJ2166,2020JJ5322)
湖南省重点研发项目(2022NK2041)
长沙市自然科学基金(kq2014173)。
关键词
凝结芽孢杆菌
液体分批发酵
菌体数
可溶性总糖
动力学方程
Bacillus coagulans
liquid fermentation in batches
viable bacterium count
soluble total sugar
kinetic equations
