摘要
目的探讨保健食品提高缺氧耐受力功能替代性新试验方法的可行性。方法采用小鼠耐缺氧实验,建立斑马鱼缺氧模型,并检测样品对斑马鱼缺氧运动的改善情况及抗缺氧诱发的红细胞增多症。以连二亚硫酸钠处理原代培养乳鼠心肌细胞构建了化学性缺氧细胞模型,样品处理后进行细胞活性与乳酸脱氢酶(LDH)活性检测,分别验证样品对缺氧耐受能力的影响。结果与正常对照比较,样品可以提高小鼠的缺氧耐受力;与缺氧模型对照比较,可以改善斑马鱼缺氧运动障碍、缺氧诱发的红细胞增多症,可以缓解心肌细胞缺氧导致的细胞活性降低及LDH活性升高。结论用体内外三种试验体系均能检出样品具有提高缺氧耐受的能力,耐缺氧检验的动物试验替代方法的推出具有可行性。
Objective To explore the feasibility of new test methods for evaluation of improving hypoxia tolerance function in health food.Methods Hypoxia tolerance experiments were carried out on mice.The zebrafish hypoxia model was established,and the improvement of zebrafish hypoxia movement and erythrocytosis were tested.A chemical hypoxia cell model was constructed with sodium disulfite,and cell activity and lactate dehydrogenase(LDH)activity were detected to verify their effect on hypoxia tolerance.Results Compared with normal control group,the hypoxia tolerance was improved in mice by health food sample.Compared with hypoxia model control groups,the sample improved zebrafish hypoxia,hypoxia-induced erycytosis,and alleviated the reduced cellular activity and increased LDH activity caused by cardiomyocyte hypoxia.Conclusion The ability of the sample to improve hypoxia tolerance can be detected by three test systems in vitro as well as in vivo,and the introduction of alternative methods to animal testing for hypoxia tolerance tests is feasible.
作者
杨隽
刘蕾
张大超
沈佳琪
黄燕烽
陶功华
洪新宇
YANG Jun;LIU Lei;ZHANG Dachao;SHEN Jiaqi;HUANG Yanfeng;TAO Gonghua;HONG Xinyu(Shanghai Municipal Center for Disease Control and Prevention,Shanghai 200336,China;China Health Association,Beijing 100142,China;Hunter Biotechnology,Inc.,Zhejiang Hangzhou 310051,China)
出处
《中国食品卫生杂志》
CSCD
北大核心
2023年第2期179-183,共5页
Chinese Journal of Food Hygiene
基金
市场监督管理总局特殊食品司项目。
关键词
小鼠
斑马鱼
心肌细胞
耐缺氧
Mouse
zebrafish
cardiomyocytes
hypoxia tolerance
