摘要
[目的]挖掘大豆Glycine max MADS转录因子家族成员GmMADS4基因信息,分析其结构及功能。[方法]通过生物信息学分析,对GmMADS4基因进行基因结构、编码蛋白信息、保守结构域、系统进化树以及互作蛋白预测等分析。利用烟草叶片瞬时转化法分析亚细胞定位,通过RT-qPCR进行组织部位及响应缺素的表达模式分析,利用下胚轴复合植株转化法分析超量表达GmMADS4对转基因毛根生长的影响。[结果]GmMADS4基因开放阅读框长732 bp,编码蛋白相对分子质量为28 000;保守结构域含有MADS-box和K-box,属于II型MADS家族成员,与拟南芥的AtAP3相似性较高;GmMADS4在大豆多个部位均有表达,且在花和种子中的表达量较高;缺氮和缺磷处理均显著增加GmMADS4在叶和根部的表达量;GmMADS4主要定位在细胞核,超量表达GmMADS4显著增加转基因毛根的可溶性磷含量。[结论]GmMADS4属于大豆II型MADS家族成员,具有核定位功能,可能在大豆种子和花的发育过程中发挥作用,并参与大豆根部缺磷响应及磷稳态调节。
【Objective】To explore the genetic information of GmMADS4,a member of soybean(Glycine max)MADS transcription factor family,and analyze its structure and function.【Method】The structure,coding protein information,conserved domain,phylogenetic tree analysis and interaction protein prediction of GmMADS4 were analyzed by bioinformatics analysis.Transient transformation of tobacco leaves was used to analyze subcellular localization,RT-qPCR was used to analyze its expression patterns in different tissues and response to nutrient deficiency,and hypocotyl complex plant transformation method was used to analyze the effects of overexpression of GmMADS4 on transgenic hair root growth.【Result】The length of open reading frame of GmMADS4 was 732 bp and the relative molecular mass of coding protein was 28000.The conserved domain of GmMADS4 contained MADS-box and K-box,which was a member of type II MADS family,and had a high similarity with Arabidopsis AtAP3.GmMADS4 was expressed in many parts of soybean,especially in flowers and seeds.The expression of GmMADS4 in leaves and roots significantly increased by nitrogen and phosphorus deficiency induction.GmMADS4 was mainly located in the nucleus,and overexpression of GmMADS4 significantly increased soluble phosphate content in transgenic hair roots.【Conclusion】GmMADS4 is a member of soybean type II MADS family,and has nuclear localization function,which may play a role in the development of soybean seeds and flowers,as well as participate in soybean root adaptive responses to phosphorus deficiency and the regulation of phosphate homeostasis.
作者
薛迎斌
宋佳
李枭艺
李小豪
陈经烨
伍萍珍
朱胜男
刘颖
XUE Yingbin;SONG Jia;LI Xiaoyi;LI Xiaohao;CHEN Jingye;WU Pingzhen;ZHU Shengnan;LIU Ying(College of Chemistry and Environment,Guangdong Ocean University,Zhanjiang 524088,China;South China Branch,National Saline-Alkali Tolerant Rice Technology Innovation Center,Zhanjiang 524088,China;College of Coastal Agricultural Science,Guangdong Ocean University,Zhanjiang 524088,China;Life Science and Technology School,Lingnan Normal University,Zhanjiang 524048,China)
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2023年第3期420-429,共10页
Journal of South China Agricultural University
基金
国家自然科学基金(32002131)
广东省自然科学基金(2020A1515011570)
广东海洋大学“南海青年学者”项目(002029001012)。
关键词
大豆
GmMADS4
亚细胞定位
表达模式
功能分析
Soybean
GmMADS4
Subcellular localization
Expression pattern
Functional analysis
