摘要
目的:探讨不同栽培模式对粗茎秦艽产量及其根际土壤微生物多样性和次生代谢产物含量的影响。方法:以不同栽培模式粗茎秦艽及其根际土壤为研究对象,采用高通量测序技术,分析根际土壤中细菌和真菌群落组成及优势菌群和差异微生物;采用高效液相色谱法测定不同栽培模式粗茎秦艽中环烯醚萜类成分含量。结果:相较于地膜种植,采取无地膜种植与间作牡丹、白芸豆、土豆和玉米等作物后,粗茎秦艽鲜品增产16.11%~17.68%、22.48%~26.34%、29.37%~32.19%、34.82%~36.57%和35.34%~39.71%,干品增产19.75%~23.17%、25.86%~29.32%、30.18%~34.94%、35.22%~39.87%和39.72%~43.73%,龙胆苦苷、马钱苷酸、獐牙菜苷和獐牙菜苦苷4种环烯醚萜类成分总含量分别提高10.17%~37.83%、5.93%~47.44%、9.09%~28.84%和10.71%~28.57%。根际土壤中细菌和真菌群落多样性明显增高(P<0.05),其中粪壳菌纲(Sordariomycetes)、锤舌菌纲(Leotiomycetes)、银耳菌纲(Tremellomycetes)、散囊菌纲(Eurotiomycetes)、镰刀菌属(Fusarium)和支孢甁霉属(Cladophialophora)等致病菌群相对丰度降低,变形菌门(Proteobacteria)、酸杆菌门(Acidobacteria)和放线菌门(Actinobacteriota)等有益菌群比例增高且逐渐成为优势菌群。结论:粗茎秦艽不同栽培模式可影响其产量和根际土壤微生物群落多样性及环烯醚萜类成分的累积,无地膜种植和间作模式具有一定优势,可为粗茎秦艽种植提供理论参考。
Objective:To investigate the effects of different cultivation modes on the yield of Gentiana crassicaulis and its microbial diversity and secondary metabolite content in the rhizosphere soil.Method:With G.crassicaulis of different cultivation modes and its rhizosphere soil as the research objects,the composition of bacterial and fungal communities,dominant bacteria,and differential microorganisms in the rhizosphere soil were analyzed by high-throughput sequencing technology.HPLC was used to determine the content of iridoids in G.crassicaulis with different cultivation modes.Result:Compared with plastic film mulching,planting without mulch and intercropping of peony,white kidney bean,potato,and corn increased the yield of fresh products by 16.11%-17.68%,22.48%-26.34%,29.37%-32.19%,34.82%-36.57%,and 35.34%-39.71%,respectively,and increased the yield of dry products by 19.75%-23.17%,25.86%-29.32%,30.18%-34.94%,35.22%-39.87%,and 39.72%-43.73%.The total content of four iridoids,including gentiopicrin,loganic acid,sweroside,and swertiamarin,increased by 10.17%-37.83%,5.93%-47.44%,9.09%-28.84%,and 10.71%-28.57%,respectively.The diversity of bacterial and fungal communities in the rhizosphere soil increased significantly(P<0.05).The relative abundance of pathogenic bacteria such as Sordariomycetes,Leotiomycetes,Tremellomycetes,Eurotiomycetes,Fusarium,and Cladophialophora decreased,and the proportions of beneficial bacteria such as Proteobacteria,Acidobacteria,and Actinobacteriota increased and they gradually became the dominant bacteria.Conclusion:Different cultivation modes can affect the yield of G.crassicaulis and its microbial diversity and iridoid content in the rhizosphere soil.Cultivation without mulch and intercropping patterns have certain advantages,which can provide theoretical references for the planting of G.crassicaulis.
作者
加多五尼
甲使阿咪
马鸿宇
叶更生
陈晨
苟恺军
任艳
拉目加
曾锐
JIADUO Wuni;JIASHI Ami;MA Hongyu;YE Gengsheng;CHEN Chen;GOU Kaijun;REN Yan;LA Mujia;ZENG Rui(School of Pharmacy,Southwest Minzu University,Chengdu 610041,China;Institute of Qinghai-Tibetan Plateau,Southwest Minzu University,Chengdu 610041,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2023年第10期192-201,共10页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家重点研发计划项目(2017YFC1700705)
西南民族大学研究生创新型科研项目(CX2021SZ93)。
关键词
粗茎秦艽
间作
根际土壤
微生物多样性
环烯醚萜
Gentiana crassicaulis
intercropping
rhizosphere soil
microbial diversity
iridoids
