摘要
目的查明一起食用八宝粥引起中毒事件的原因,建立28种生物碱的液相色谱-串联质谱筛查方法,为此类中毒事件处置提供借鉴。方法采用液相色谱-串联质谱联用技术筛查患者尿液、八宝粥以及食物原料中28种生物碱,对生物碱进行定量检测。样品用0.4%甲酸水溶液提取,用Acquity UPLC BEH C18色谱柱(1.7μm,100×2.1 mm)分离。采用乙腈-0.2%甲酸水为流动相,梯度洗脱。电离方式为电喷雾正电离模式,检测方式采用多反应监测,外标法定量。结果在0~100 ng/mL浓度范围,28种生物碱线性相关系数r≥0.999。尿样中28种生物碱回收率为63.0%~105.0%,相对标准差(relative standard deviations,RSDs)为5.8%~8.6%;八宝粥中28种生物碱回收率为72.0%~109.0%,RSDs为6.3%~9.7%;黑芝麻中28种生物碱回收率为60.0%~95.0%,RSDs为4.8%~8.2%。患者尿液中检出莨菪碱(2380.0 ng/mL)、东莨菪碱(3.6 ng/mL)和山莨菪碱(4.7 ng/mL)。八宝粥中检出莨菪碱(63.3μg/g)、东莨菪碱(5.7μg/g)和山莨菪碱(2.1μg/g)。在原料曼陀罗籽中检出莨菪碱(901.0μg/g)、东莨菪碱(80.0μg/g)和山莨菪碱(30.1μg/g)。曼陀罗籽中东莨菪碱与莨菪碱含量的比率为1∶11,符合曼陀罗(Datura stramonium L.)特征。尿液中,东莨菪碱和山莨菪碱占比为0.15%和0.20%,莨菪碱占比为99.65%。结论种子形态、3种生物碱的含量范围以及比例,均符合曼陀罗特点,结合患者阿托品中毒临床症状,可以确定本次事件是由误食曼陀罗籽引起的一起家庭食物中毒。本研究可以为生物碱中毒患者的临床诊断和治疗提供技术支撑,为相关中毒事件应急检测和处置提供依据。
Objective To investigate a poisoning incident caused by eating eight treasure congee,and establish liquid chromatography(LC)-mass spectrometry(MS)/MS screening method of 28 alkaloids to provide references for disposal of similar poisoning incidents.Methods LC-MS/MS was used for screening 28 alkaloids in the urine,eight treasure congee and food raw material,and the detected alkaloids were quantified.Samples were extracted with 0.4%formic acid aqueous solution and separated by a Acquity UPLC BEH C18 column(1.7μm,100×2.1 mm).Acetonitrile-0.2%formic acid aqueous solution was used as the mobile phase and gradient elution was adopted.The ionization mode was electrospray positive ionization mode,and the detection method was multi-reaction monitoring(MRM).Analytes were quantified with the external standard method.Results In the concentration range of 0-100 ng/mL,the linear correlation coefficient r were greater than 0.999 for 28 alkaloids.The recovery of 28 alkaloids in urine sample ranged from 63.0%to 105.0%,and the relative standard deviations(RSDs)were between 5.8%and 8.6%.The recovery of 28 alkaloids in eight treasure congee sample ranged from 72.0%to 109.0%,and the RSDs were between 6.3%and 9.7%.The recovery of 28 alkaloids in semen sesami nigrum sample ranged from 60.0%to 95.0%,and the RSDs were between 4.8%and 8.2%.Hyoscyamine(2380.0 ng/mL),scopliamine(3.6 ng/mL)and racanisodamine(4.7 ng/mL)were detected in the patient's urine.Hyoscyamine(63.3μg/g),scopliamine(5.7μg/g)and racanisodamine(2.1μg/g)were detected in eight treasure congee.Hyoscyamine(901.0μg/g),scopliamine(80.0μg/g)and racanisodamine(30.1μg/g)were detected in the seed of Datura stramonium L.The ratio of scopliamine and hyoscyamine in the seed of D.stramonium was 1∶11,which complies with the characteristics of D.stramonium L.In urine sample,the proportion of scopliamine and rac-anisodamine was 0.15%and 0.20%,and hyoscyamine accounted for 99.65%.Conclusion Seed morphology,the content range and proportion of three alkaloids are all in accord with the characteristics of D.stramonium.Combined with the clinical symptoms of atropine poisoning,it can be deduced that this incident is a family food poisoning caused by accidental consumption of seed of D.stramonium L.The method can provide technical support for the clinical diagnosis and treatment of alkaloid poisoning patients,and also provide a basis for emergency detection and disposal of alkaloid poisoning events.
作者
赵凌国
罗兰
尹臻一
任燕
雷蕾
马志锋
ZHAO Ling-guo;LUO Lan;YIN Zhen-yi;REN Yan;LEI Lei;MA Zhi-feng(Baoan District Center for Disease Prevention and Control,Shenzhen,Guangdong 518101,China;Longhua District Center for Disease Prevention and Control,Shenzhen,Guangdong 518110,China;School of Public Health,Chongqing Medical University,Chongqing 400016,China)
出处
《中国热带医学》
CAS
2023年第3期260-266,共7页
China Tropical Medicine
基金
广东省医学科学技术研究基金项目(No.A2021416)。
关键词
生物碱
食物中毒
液相色谱-串联质谱法
曼陀罗
莨菪碱
东莨菪碱
山莨菪碱
快速筛查
Alkaloids
food poisoning
liquid chromatography-tandem mass spectrometry
Datura stramonium Linn
hyoscyamine
scopliamine
rac-anisodamine
screening
