摘要
目的:探讨蒲公英提取物对类风湿性关节炎成纤维样滑膜(FLS)细胞增殖、凋亡及炎症的影响及其可能作用机制。方法:原代分离培养类风湿性关节炎成纤维样滑膜细胞,不同浓度的蒲公英提取物处理成纤维样滑膜细胞,检测细胞增殖、凋亡及IL-6、TNF-α;qRT-PCR法检测miR-708-5p的表达量;miR-NC、miR-708-5p mimics分别转染至成纤维样滑膜细胞,anti-miR-NC、anti-miR-708-5p分别转染至成纤维样滑膜细胞后加入5 mg/mL蒲公英提取物培养细胞,检测细胞增殖、凋亡及IL-6、TNF-α。结果:蒲公英提取物处理后细胞活力和IL-6、TNF-α的水平降低(P<0.05),miR-708-5p的表达量、细胞凋亡率升高(P<0.05);转染miR-708-5p mimics可降低细胞活力和IL-6、TNF-α的水平(P<0.05),细胞凋亡率升高(P<0.05);转染anti-miR-708-5p可减弱蒲公英提取物对成纤维样滑膜细胞增殖、凋亡及炎症反应的作用。结论:蒲公英提取物可通过促进miR-708-5p表达而抑制类风湿性关节炎成纤维样滑膜细胞增殖、炎症反应并诱导细胞凋亡。
Objective:To explore the efficacy of dandelion extract on the proliferation,apoptosis and inflammation of fibroblast-like synovial cells in rheumatoid arthritis and its possible mechanism.Methods:Primary isolation and culture of rheumatoid arthritis fibroblast-like synovial(FLS)cells was performed,and the FLS cells were treated with different concentrations of dandelion extract.The cell proliferation,apoptosis and IL-6 and TNF-αwere detected.The qRT-PCR method was used to detect the expression of miR-708-5p.The miR-NC and miR-708-5p mimics were transfected into FLS cells;anti-miR-NC and anti-miR-708-5p were transfected into FLS cells respectively,and 5 mg/mL dandelion extract was added to culture cells.The above methods were used to detect cell proliferation,apoptosis and the levels of IL-6 and TNF-α.Results:After dandelion extract treatment,cell viability and the levels of IL-6,TNF-αwere decreased(P<0.05),while the expression of miR-708-5p,cell apoptosis rate were increased(P<0.05).Transfection of miR-708-5p mimics could reduce cell viability and the levels of IL-6,TNF-α(P<0.05),while cell apoptosis was increased(P<0.05).Transfection of anti-miR-708-5p could attenuate the effects of dandelion extract on the proliferation,apoptosis and inflammation of FLS cells.Conclusion:Dandelion extract could inhibit rheumatoid arthritis fibroblast-like synovial cell proliferation,inflammation and induce cell apoptosis by promoting the expression of miR-708-5p.
作者
王亚男
李艳
武文印
王子
WANG Yanan;LI Yan;WU Wenyin;WANG Zi(Rheumatism Immunometabolism Ward,Tangshan Hospital of Traditional Chinese Medicine,Tangshan 063000,Hebei China;Department of Preventive Medicine,Tangshan Hospital of Traditional Chinese Medicine,Tangshan 063000,Hebei China.)
出处
《中国中医骨伤科杂志》
CAS
2023年第4期9-14,共6页
Chinese Journal of Traditional Medical Traumatology & Orthopedics
基金
河北省中医药管理局科研计划项目(2017129)。
