摘要
[目的]探讨螺旋拟柱孢藻藻株是否具有潜在的抑菌活性。[方法]从汕头澄海人工对虾养殖池分离纯化藻株,通气扩大培养、离心收集、冷冻干燥后,采用乙醇提取并用柱层析分离,先后得到藻体的乙醇提取物和叶绿素提取物。以金黄色葡萄球菌和大肠杆菌为受试菌种,采用圆形纸片法进行抑菌活性研究。[结果]藻体乙醇提取物对金黄色葡萄球菌和大肠杆菌有抑菌作用,其抑菌直径分别为11.8、10.3 mm;藻叶绿素粗提物仅对金黄色葡萄球菌有抑菌作用,抑菌直径为24.2 mm。[结论]螺旋拟柱孢藻乙醇提取物对金黄色葡萄球菌和大肠杆菌具有中等强度的抑菌活性,而藻体叶绿素提取物仅对金黄色葡萄球菌抑菌,但呈高灵敏抑菌效应,该藻株有望成为生物抑菌来源物质的新型材料而得到进一步开发利用。
[Objective]To explore the potential antibacterial activity of Cyanobacteria C.raciborskii helix.[Method]After isolation and purification of the algae from the artificial shrimp culture pond in Chenghai,Shantou,aeration expansion culture,centrifugal collection,freeze-drying,ethanol extraction and column chromatography separation were used to obtain the ethanol extract and chlorophyll pigment extract of the algae.The antibacterial activity of Staphylococcus aureus and Escherichia coli was studied by circular disk method.[Result]The ethanol extract of the algal body had bacteriostatic effect on Staphylococcus aureus and Escherichia coli,and its bacteriostatic diameter was 11.8 mm and 10.3 mm respectively;The crude extract of algal chlorophyll only had bacteriostatic effect on Staphylococcus aureus,and the bacteriostatic diameter was 24.2 mm.[Conclusion]The ethanol extract of C.raciborskii had moderate antibacterial activity against S.aureus and E.coli,while chlorophyll extract only inhibited S.aureus with strong activity.It was expected to be a new source material of biological antibacterial and be further developed and utilized.
作者
聂利华
张宇超
钟富胜
肖璐
郑雅琼
NIE Li-hua;ZHANG Yu-chao;ZHONG Fu-sheng(Department of Bioengineering,Jieyang Polytechnic,Jieyang,Guangdong 522000)
出处
《安徽农业科学》
CAS
2023年第8期1-5,共5页
Journal of Anhui Agricultural Sciences
基金
揭阳职业技术学院创新强校工程(2016-2020)立项项目(JYPSHFW_B1701)
揭阳职业技术学院校级大学生创新创业训练项目(JYCXLJH1902)
揭阳市科技计划项目(2019ny011)
广东省科技创新战略专项资金(pdjh2022b1078)。
关键词
蓝细菌
螺旋拟柱孢藻
乙醇提取物
叶绿素提取物
抑菌活性
Cyanobacteria
Cylindrospermopsis raciborskii helix
Ethanol extract
Chlorophyll extract
Antibacterial activity
