摘要
目的观察雷公藤甲素(TP)对哮喘小鼠气道炎症损伤的保护作用,并探讨其作用机制。方法取30只健康雌性BALB/c小鼠随机分为对照组、模型组和TP组(40μg/kg),每组10只。模型组和TP组采用卵清白蛋白(OVA)致敏及激发诱导小鼠哮喘模型;TP组小鼠于每次激发前经腹腔注射40μg/kg TP,对照组和模型组小鼠均予以等体积PBS溶液注射。末次激发24 h后检测气道高反应性;通过苏木素-伊红(HE)和过碘酸(PAS)染色观察小鼠气道病理改变;收集支气管肺泡灌洗液(BALF),计数嗜酸性粒细胞(EOS)、淋巴细胞(Lym)及中性粒细胞(NEU),采用ELISA法检测白介素-10(IL-10)、白介素-17(IL-17)含量水平,采用流式细胞术检测辅助性T细胞17(Th17)、调节性T细胞(Treg)细胞数量。结果与对照组比较,模型组小鼠气道高反应性增加(P<0.05),气道炎性浸润及黏液分泌增加,BALF中EOS、Lym、NEU、Th17细胞百分比和IL-17水平显著升高(P<0.05),BALF中Treg细胞百分比和IL-10水平显著下降(P<0.05)。与模型组比较,TP组小鼠气道高反应性降低(P<0.05),炎性浸润及黏液分泌减少,BALF中EOS、Lym、NEU、Th17细胞百分比和IL-17水平显著下降(P<0.05),BALF中Treg细胞百分比和IL-10水平显著增加(P<0.05)。结论TP可能通过调节Th17/Treg平衡,改善哮喘小鼠气道炎症和气道高反应性。
Objective To observe the protective effect of triptolide(TP)from airway inflammation in mice with asthma and explore its mechanism.Methods A total of 30 healthy female BALB/c mice were enrolled and randomly divided into control group,model group and TP group(40μg/kg),with 10 cases in each group.The mice were sensitized and stimulated by ovalbumin(OVA)to induce the asthma model in model group and TP group.Before each stimulation,mice in TP group were intraperitoneally injected with 40μg/kg TP,while mice in control group and model group were injected with the same volume of PBS solution.At 24 h after the last stimulation,airway hyperresponsiveness was detected.The pathological changes of airway were observed by hematoxylin-eosin(HE)and periodic acid(PAS)staining.The bronchoalveolar lavage fluid(BALF)was collected to count eosinophils(EOS),lymphocytes(Lym)and neutrophils(NEU).The levels of interleukin-10(IL-10)and interleukin-17(IL-17)were detected by ELISA.The numbers of helper T cells 17(Th17)and regulatory T cells(Treg)were detected by flow cytometry.Results Compared with control group,the airway hyperresponsiveness of mice was increased in model group(P<0.05),the airway inflammatory infiltration and the mucus secretion were increased,the percentages of EOS,Lym,NEU and Th17,and IL-17 level in BALF were increased(P<0.05),and the percentage of Treg and IL-10 level in BALF were decreased in model group(P<0.05).Compared with model group,the airway hyperresponsiveness of mice was decreased in TP group(P<0.05),the inflammatory infiltration and the mucus secretion were reduced,the percentages of EOS,Lym,NEU and Th17,and IL-17 level in BALF were decreased(P<0.05),and the percentage of Treg and IL-10 level in BALF were increased in TP group(P<0.05).Conclusion TP may improve the airway inflammation and the hyperrespon-siveness in mice with asthma by regulating Th17/Treg balance.
作者
林爽
罗彬
陈丽萍
LIN Shuang;LUO Bin;CHEN Liping(Department of Emergency,Fourth Affiliated Hospital of Xinjiang Medical University,Urumqi 830000,China;Department of Critical Care Medicine,Fifth Affiliated Hospital of Xinjiang Medical University;Department of Critical Care Medicine,Xinjiang Uygur Autonomous Region People’s Hospital)
出处
《山西医科大学学报》
CAS
2023年第3期322-326,共5页
Journal of Shanxi Medical University
基金
新疆维吾尔自治区自然科学基金项目(2016D01C112)。
