摘要
分析汾河太原段沉积物细菌群落结构,探讨细菌群落与典型环境因子的相关关系,丰富河流沉积物细菌群落资料,并为汾河太原段生态健康状况评价提供依据。于2020年10月枯水期,在汾河入城口至清徐段布设11个采样断面,采集汾河太原段表层沉积物,共采集到10份样本。采用Illumina高通量测序技术分析沉积物细菌丰度和群落结构,利用主成分分析(PCoA)、冗余分析(RDA)和相关性分析等方法探讨沉积物中细菌群落结构分布特征以及主要影响因素。结果表明,10个断面表层沉积物中5 422个细菌OTUs分属于61门140纲346目580科1 073属。细菌群落结构在断面fh6、fh7、fh8、fh9、fh10、fh11和fh4相似度较高,fh1和fh2断面相似度较高,而fh3断面与其他断面差异显著。除fh3,沉积物细菌优势菌门均为变形杆菌门(Proteobacteria),次优势菌门为绿弯菌门(Chloroflexi),优势菌纲为γ-变形菌纲(Gammaproteobacteria)。Pearson相关性分析表明,TN、OM和Pb与OTUs、Shannon指数和Simpson指数相关性显著。TN、OM和Pb是细菌群落结构的主要影响因子。RDA和Spearman分析表明,变形杆菌门(Proteobacteria)丰度与TN和OM呈显著正相关(P<0.01),与Pb和Ni呈正相关(P<0.05);绿弯菌门(Chloroflexi)与Cr呈显著负相关(P<0.01),与OTC和TC呈显著正相关(P<0.01);放线菌门(Actinobacteria)与TN、OM和Cu呈负相关(P<0.05);厚壁菌门(Firmicutes)与pH呈负相关,与TP呈正相关(P<0.05)。
Fenhe River is the primary water source for industry,agriculture and domestic use in Shanxi Province.Conserving the ecological environment of the Taiyuan section of Fenhe River is significant not only for the quality life in Taiyuan city but also for protecting downstream water quality.In this study,we explored the bacterial community structure and its relationship with sediment environmental factors in the Taiyuan section of Fenhe River.In October 2020,sediment samples were collected at 11 transects of the river section(fh1-fh5 in the suburban area and fh6-fh11 in the urban area)for analysis of the bacterial community and determination of physiochemical parameters,heavy metals and antibiotics(no samples were obtained at site fh5).Illumina high-throughput sequencing technology was used to analyze bacterial abundance and community structure in surface sediments,and principal co-ordinates analysis(PCoA),redundancy analysis(RDA)and correlation analysis were applied to explore the distribution of the bacterial community and primary influencing factors.A total of 5422 bacterial OTUs belonging to 1073 genera,580 families,346 orders,140 classes and 61 phyla were obtained in surface sediments of the 10 transects.Across the 10 transects the dominant phyla at all transects,except for fh3,was Proteobacteria(40.63%)and other prevalent phyla included Chloroflexi(18.41%),Actinobacteria(9.84%),Bacteroidetes(7.49%),Acidobacteria(6.46%)and Firmicutes(4.80%).The dominant class among the bacterial communities was Gammaproteobacteria.The Shannon,Simpson,Chao and Ace indices of bacterial diversity were in the respective ranges of 5.82-6.88,0.0026-0.0125,3026-3240 and 3089-3597.The similarity of bacterial community structure at sites fh6,fh7,fh8,fh9,fh10,fh11 and fh4 was high,clustering into one group,with fh1 and fh2 clustering into a second group,but there were significant differences in community structure between fh3 and the other transects.Pearson correlation analysis shows that total nitrogen(TN),organic matter(OM)and lead(Pb)were significantly correlated with the OTUs,and the Shannon and Simpson indices,indicating that they were the primary factors affecting bacterial community structure.Redundancy analysis and Spearman analysis show that Proteobacteria had a highly significantly positive correlation with TN and OM(P<0.01),and significant positive correlations with Pb and Ni(P<0.05);Chloroflexi was significantly negatively correlated with Cr(P<0.01)while significantly positively correlated with OTC and TC(P<0.01);Actinobacteria was negatively correlated with TN,OM and Cu(P<0.05).Firmicutes was negatively correlated with pH and positively correlated with TP(P<0.05).The results of this study provide data that will improve our understanding of the bacterial community distribution in the sediments of the Taiyuan section of Fenhe River and identified the environmental impact factors.
作者
王倩
甄志磊
王春玲
马浩天
孙镱铭
曹凯丽
WANG Qian;ZHEN Zhi-lei;WANG Chun-ling;MA Hao-tian;SUN Yi-ming;CAO Kai-li(College of Urban and Rural Construction,Shanxi Agricultural University,Jinzhong 030801,P.R.China)
出处
《水生态学杂志》
CSCD
北大核心
2023年第3期110-119,共10页
Journal of Hydroecology
基金
山西省高等学校科技创新项目(2020L0143)
山西农业大学科技创新基金(2017YJ04)。
关键词
沉积物
细菌群落
重金属
抗生素
汾河
sediment
bacterial community
heavy metals
antibiotics
Fenhe River
